Bioremoval of Contaminated in Wastewater by Using Alginate Immobilized Cell of Green Microalga Nannochloropsis oculata
Main Article Content
Abstract
The optimum conditions and adsorption kinetic for bioremoval of benefix red by immobilized cell of Nannochloropsis oculata were studied. The results showed that the optimum pH for bioremoval of dye was at 2 with the highest adsorption capacity of 0.90±0.01 mg/g. The equilibrium time of adsorption was 2 h with adsorption capacity of 1.80±0.02 mg/g. The optimum biomass was 2 g/l with adsorption capacity of 3.98±0.02 mg/g. The adsorption capacity (qeq) decreased when the algal biomass increased. The adsorption characteristics fitted well with the Langmuir adsorption isotherm with maximum adsorption capacity (Qmax) of 106.58±20.22 mg/g. Dye removal correlated with pseudo first-order, while the adsorption rate was limited by film and intraparticle diffusion at secondary step. It can be concluded that alginate immobilized cell of N. oculata may be used as an alternative for removal of benefix red contaminated in wastewater.
Article Details

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
King Mongkut's Agricultural Journal
References
Akzu, Z., & Tezer, S. (2005). Biosorption of reactive dyes on the green alga Chlorella vulgaris. Process Biochemistry, 40(3-4), 1347-1361. https://doi.org/10.1016/j.procbio.2004.06.007
Aldegs, Y., Elbarghouthi, M., Elsheikh, A., & Walker, G. (2008). Effect of solution pH, ionic strength, and temperature on adsorption behavior of reactive dyes on activated carbon. Dyes and Pigments, 77(1), 16-23. https://doi.org/10.1016/j.dyepig.2007.03.001
Buhani, Suharso, Miftahza, N., Permatasari, D., & Sumadi. (2021a). Improved adsorption capacity of Nannochloropsis sp. through modification with cetyltrimethylammonium bromide on the removal of methyl orange in solution. Adsorption Science & Technology, 2021(9), 1-14. https://doi.org/10.1155/2021/1641074
Buhani, Wijayanti, T. A., Suharso, Sumadi, & Ansori, M. (2021b). Application of modified green algae Nannochloropsis sp. as adsorbent in the simultaneous adsorption of methylene blue and Cu(II) cations in solution. Sustainable Environment Research, 31(1), 1-12. https://doi.org/10.1186/s42834-021-00090-y
Celekil, A., Yavuzatmaca, M., & Bozkurt, H. (2012). Binary adsorption of reactive red 120 and yellow 81 on Spirogyra majuscula. Middle East Journal of Scientific Research, 1(2), 29-36. https://doi.org/10.5829/idosi.mejsr.2013.13.6.2316
Gunasundari, E., Kumar, P. S., Rajamohan, N., & Vellaichamy, P. (2020). Feasibility of naphthol green-B dye adsorption using microalgae: thermodynamic and kinetic analysis. Desalination and Water Treatment, 192(2020), 358-370. https://doi.org/10.5004/dwt.2020.25777
IBM SPSS Statistics. (2023). KMITL SPSS Version29. Retrieved from: https://drive.google.com/file/d/1FDY_FmOy1PgwB3l7ARGBA-6XJy84kcBW/view?usp=sharing.
Kumar, S., Ahluwalia, A. S., & Charaya, M. U. (2019). Adsorption of orange-g dye by the dried powdered biomass of Chlorella vulgaris Beijerinck. Current Science, 116(4), 604-611. https://doi.org/10.18520/cs/v116/i4/604-611
Neag, E. & Roman, C. (2017). Applications of microalgae in wastewater treatment experimental and equilibrium studies. In Proceedings of 2017 International Conference on Hydraulics and Pneumatics – HERVEX, pp.318-324. Institute for Analytical Instrumentation Russian Academy of Sciences.
Ozer, A., Ozer, D., & Eklz, H. I. (2005). The Equilibrium and Kinetic Modelling of the Biosorption of Copper(II) Ions on Cladophora crispata. Adsorption, 10(4), 317-326. https://doi.org/10.1007/s10450-005-4817-y
Radwan, E. K., Abdel-Aty, A. M., El-Wakeel, S. T., & Abdel Ghafar, H. H. (2020). Bioremediation of potentially toxic metal and reactive dye-contaminated water by pristine and modified Chlorella vulgaris. Environmental Science and Pollution Research, 27(17), 21777-21789. https://doi.org/10.1007/s11356-020-08550-5
Ruangsomboon, S., & Chonudomkul, D. (2022). Effect of CO2 concentration on growth, CO2 fixation, and biochemical composition of the microalga Nannochloropsis oculata. Chiang Mai Journal of Science, 49(2), 325-338. https://doi.org/10.12982/CMJS.2022.035
Sani, Z. M., Dalhatu, A. S., Adam, B. S., Mohammed, K., Muhammad, Y. Y., & Ibrahim, S. (2021). Bioremediation of some reactive dyes commonly used in Fabric re-dyeing by Chlorella vulgaris. Asian Journal of Environment & Ecology, 15(4), 10-19.
Sarwa, P., Vijayakumar, R., & Verma, S. K. (2014). Adsorption of acid red 66 dye from aqueous solution by green microalgae Acutodesmus obliquus strain PSV2 isolated from an industrial polluted site. Open Access Library Journal, 1(3), 1-8. https://doi.org/10.4236/oalib.1100712
Schiewer, S., & Volesky, B. (2000). Biosorption Processes for Heavy Metal Removal. In Lovley, D.R. (Ed.) Environmental Microbe-Metal Interactions, pp.329-362. American Society for Microbiology.
Vojnovic, B., Cetina, M., Franjkovic, P., & Sutlovic, A. (2022). Influence of initial pH value on the adsorption of reactive black 5 dye on powdered activated carbon: kinetics, mechanisms, and thermodynamic. Molecules, 27(4), 1-12. https://doi.org/10.3390/molecules27041349
Yemendzhiev, H., Alexieva, Z., & Krastanov, A. (2014). Decolorization of Synthetic Dye Reactive Blue 4 by Mycelial Culture of White - Rot Fungi Trametes Versicolor 1. Biotechnology & Biotechnological Equipment, 23(3), 1337-1339.