Effect of Nutrient Concentration in MS Strength on Growth and Antioxidant Properties in Bacopa monnieri (L.) Wettst. Growing in In Vitro Cultures
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Abstract
Bacopa monnieri (L.) Wettst. is a medical plant that contains a large number of metabolites including alkaloids, glycosides, flavonoids and saponins, which are compounds that have a wide range of therapeutic properties including antioxidant activities. Saponin is the main compound found in B. monnieri (L.) Wettst. This study was to do with the effects of Murashige and Skoog (MS) medium that contained 4 different nutrition concentrations, namely ¼ MS, ½ MS, MS and 2 MS, on the growth and antioxidant activity of B. monnieri (L.) Wettst. grown in in vitro. The ¼ MS treatment emerged as the most effective promoter of growth and development as explants grown on it reached the experimental maximum height of 45.50 mm per explant, the highest number of leaves at 18.5 leaves per explant, the highest number of nodes at 8.73 nodes per explant, the highest number of roots at 6.53 roots per explant and the highest values of dry and fresh weight. However, across all treatments, namely ¼ MS, ½ MS, MS and 2 MS, there were no statistically significant differences in the number of shoots (P>0.05) produced. The study also demonstrated that the ¼ MS treatment led to the highest concentrations of TPC, TFC and antioxidant activity by ABTS assay (19.32 mg gallic/g, 43.89 mg luteolin/g and 88.28 % respectively). Significantly, the control treatment (MS) produced the highest saponin concentration, which was 24,666.81 mg saponin/g. Finally, no significant differences with respect to DPPH assay were found among the various treatments undertaken in this research (P>0.05).
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King Mongkut's Agricultural Journal
References
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