Efficacy of Endophytic Bacteria on Growth Inhibition of Important Plant Pathogenic Fungi
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Abstract
Endophytic bacteria are one type of microorganism that can be used to effectively control plant-pathogenic fungi. However, the use of these microorganisms in plant production improvement is still limited because of problems with levels of efficacy and maintenance or stability of efficacy. In order to study efficacy, experiments were conducted to test the viability of endophytic bacteria isolated from tomato (SuRW02, SuRW01 and LbRW03) and rice (Su2S217, SuR317, BaS417 and BaR917), isolates which were previously reported to be able to control Fusarium sp. and Pyricularia sp. We also studied the ability of the bacterial isolates to maintain their efficiency as antagonist microorganisms after long-time storage. Included was a dual culture test with other plant pathogenic microorganisms, performed in order to observe additional antagonistic activity. The results showed that all isolates survived, and some isolates were able to inhibit the growth of other fungal pathogens. The isolates LbRW03, SuRW01 and SuRW02 isolated from tomato not only showed the ability to inhibit Fusarium oxysporum sp. growth; they also were able to inhibit Pyricularia sp. and C. capsici. With regard to rice endophytic bacteria, it was found that some isolates had inhibitory effects on specific hosts; however, SuR317 was able to inhibit C. capsici,
F. oxysporum and Pythium sp. Two isolates, BaR917 and SuRW02, were selected for 16s rRNA gene sequence studies. The results indicated that the isolates belonged to Bacillus and Sphingobacterium, respectively.
Article Details
King Mongkut's Agricultural Journal
References
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