<I>In Vitro </I> and <I>In Vivo </I> Propagation of <I> Chlorophytum undulatum </I> Wall

Abstract

An in vitro  production of Chlorophytum undulatum Wall. has been established. It was found that culturing pseudostem explants on modified MS medium without any growth regulator produced 1.30 shoots/explant within 1 month. Explants on the same medium + 0.5 mg/l Thidiazuron (TDZ) produced 2.25 shoots/explant. Continuously culturing explants on media supplemented with TDZ for 5 months, with 1 month interval of subculturing onto fresh medium, gave the highest yield of 2.80 shoots/explant. However, continuously culturing on media with TDZ for more than 5 months lowered the number of shoots to 2.41 shoots/explant at month 7. A study on different in vivo media components was also carried out.  It was found that plants in loamy soil + sand + charred rice husk, 1:1:1 ratio, had the highest height, leaf width, and leaf length i.e. 17.03, 2.11 and 11.30 cm, respectively. Plants in loamy soil or loamy soil + cow manure, 1:1 ratio, produced the highest number of shoot, 4.14 shoots in 2 months.