Efficacy of Antagonistic Fungi <I> Trichoderma </I> spp. in Controlling Soybean Seedling Anthracnose

Abstract

The antagonistic fungi, Trichoderma spp. were isolated from soybean seeds  cultivars CM 60, SJ 5, MJ 9518-2 and MJ 9520-21 by blotter method. Four isolates of Trichoderma spp. were obtained from soybean cultivars CM 60, SJ 5 and MJ 9520-21. These Trichoderma spp. were tested for antagonistic activities using dual culture method against Colletotrichum truncatum which caused anthracnose disease of soybean. All isolates of Trichoderma spp. had shown high percentage of growth inhibition. On the slide dual culture technique, Trichoderma spp. showed mycoparasitic characters by coiling around and penetrating into C. truncatum  hyphae. The efficacies of the four isolates of Trichoderma spp. and captan fungicide in controlling anthracnose on soybean seedlings  cultivar CM 60 were examined under the greenhouse condition. The results showed that all isolates  of Trichoderma spp. and captan had reduced the anthracnose infection on the tested soybean seedlings. In contrast, captan statistically gave more effectiveness in controlling of anthracnose on soybean seedlings than all isolates of Trichoderma spp..