Phylogenetics of the Powdery Mildews in Tribe Phyllactinieae in Northern Thailand

Abstract

The morphological study of the powdery mildews in Tribe Phyllactinieae in Northern Thailand and phylogenetic  relationship of  this fungal group, 13 fungal specimens, were used for sequencing of a nuclear large subunit (28S) and internal transcribed spacer (ITS) of  rDNA region and compared with the sequence obtained from the DNA Databank of Japan (DDBJ). The sequences were initially aligned using the Clustal X package. Phylogenetic tree was obtained from the data using maximum parsimony methods. The results indicated that 9 specimens were belonging to genus Ovulariopsis and 4 specimens were belonging  to genus Oidiopsis. Moreover, from the phylogenetic tree, 9 fungi in genus Ovulariopsis can be grouped into 6 different groups. The genus Oidiopsis was classified as the same fungal group and are identified as Leveillula taurica. In addition, within 15 host plants species, 13 species were first recorded in Thailand, in which 2 of  these were first recorded in the world.  This was the first comprehensive study of the powdery mildews belonging to Tribe Phyllactinieae in Thailand which compared morphological characteristics and phylogenetic analyses of  the ITS and 28S nucleotide sequence.