Screening SSR Markers for Brown Planthopper Resistant Genes, <I>Bph3</I>, on Rice (<I>Oryza indica</I> L.) Introgression Lines and Chai Nat 1

Abstract

Currently molecular markers used to determine any resistant gene of brown planthopper (Nilaparvata lugens (Stål), Delphacidae, Homoptera) were developed rapidly and variously available which provided different results depended on the varieties of rice. Therefore, the screening suitable SSR markers to determine brown planthopper resistant genes, Bph3, on rice (Oryza indica L.) introgresion lines of Rathu Heenati/KDML 105 and Chai Nat 1 was carried out in order to use for improvement of brown planthopper resistant trait of Chai Nat 1. Seven potential SSR markers located on chromosome 6 closed to Bph3 resistant gene were used to determine Bph3 resistant gene composed of RM 170, RM 190, RM 586, RM 587, RM 588, RM 589, and RM 597. However, only RM 170, RM 190, and RM 586 were presented the best evident of polymorphism result with 10-15 nucleotide bases differences between both target rice varieties and clearly enough to use for following and evaluation on the existence of Bph3 resistant gene in the process of  Chai Nat 1 improvement in the future.