<I>IN VITRO</I> STORAGE OF ZYGOTIC EMBRYO OF RUBBER (<I>HEVEA BRASILIENSIS</I> MUELL ARG.) AT LOW TEMPERATURE
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Oct 29, 2020
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Abstract
Zygotic embryos of local cultivar were stored on Murashige & Skoog (MS) free medium supplemented with 3% sucrose and 2 kinds of cryoprotectants, Mannitol and Sorbitol. The cultures were maintained under 2,000 lux illumination, 14 hour photoperiod at 10 ± 2 °C. By this method, it was found that zygotic embryos could be stored on those media for 6 weeks. Between the two cryoprotectants tested, Mannitol gave the highest percent germination after storage significantly different from that obtained on the medium with Sorbitol. Percent germination after on Mannitol and Sorbitol were 72.96 and 57.00, respectively.
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References
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Deabrunais, B.A., Noirot, M. and Charrier, A. (1991). Minimal growth invitro conservation of coffee (Coffea spp.) 1. Influence of low concentrations of 6-benzyladenine. Plant Cell, Tissue and Organ Culture, 27: 333-339.
Fukai, S. (1990). Cryopreservation of chrysanthemum shoot tips. Scientia Horticulturae. 46: 167–174.
Hariher, G. and Ying, H.Y. (1984). The low fruit set that fallows conventional hand pollination in Hevea brasilicasis: insufficiency of pollen as a couse. RRIM Journal. 32: 20-29.
Ko, W., Hwang, S. and Ku, F. (1991). A new technique for storage of meristem-tip cultures of Cavendish banana. Plant Cell, Tissue and Organ Culture. 25: 179-183.
Reed, M.B. (1990). Survival of in vitro grown apical meristem of pyrus following cryopreservation. Hort Science. 25: 111-113.
Sharma, K.K. (1990). In vitro propagation of Malus alba through nodal segment. ScientiaHorticulturae. 42: 307-320.
Ziauddin, A. and Kasha, J.K. (1990). Long-term callus cultures of diploid Barley (Hordeum vulgare). l. Auxin effects on culture initiation and maintenance. Euphytica, 48: 171-176.
