การประเมินประสิทธิภาพการสืบพันธุ์ของโคนมโดยวิธีเรดิโออิมมูนโนแอสเซ
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พ.ย. 9, 2020
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บทคัดย่อ
ทำการเก็บตัวอย่างน้ำนม จากโคนมในจังหวัดเชียงใหม่ ในวันที่ 22, 42 และ 64 หลังวันผสมเทียมเพื่อวิเคราะห์ หาระดับฮอร์โมนโปรเจสเตอร์โรน (Progesterone) โดยวิธีเรดิโออิมมูนโนแอสเซ (Radioimmunoassay, RIA) โคส่วนใหญ่เป็นลูกผสมระหว่างพันธุ์พื้นเมืองและโฮสไสตน์-ฟรีเชี่ยน (Holstein-Friesian) โดยมีเลือดโคพันธุ์โฮสไสตน์ฟรีเชี่ยน อยู่ระหว่าง 50 ถึง มากกว่า 80 เปอร์เซนต์ วัดระดับฮอร์โมน โปรเจสเตอร์โรนในน้ำนมของโคที่ไม่ตั้งครรภ์ได้ 4.25, 3.70 และ 3.58 นนก./มล. ส่วนโคกลุ่มที่ตั้งครรภ์วัดได้ 0.78, 11.47 และ 11.08 นนก. / มล. ในวันที่ 22, 42 และ 64 หลังวันผสมพันธุ์ตามลำดับ ความแม่นยำในการวัดสภาวะไม่ตั้งครรภ์ และ ตั้งครรภ์ ในวันที่ 22 หลังวันผสมพันธุ์มีค่า 90.9 และ 82.8 วันที่ 42 หลังวันผสมพันธุ์มีค่า 50.0 และ 96.4 วันที่ 64 หลังวันผสมพันธุ์มีค่า 100 และ 92.3 เปอร์เซนต์ ตามลำดับ อัตราการตั้งท้องของโคมีค่าเฉลี่ย 67.3 เปอร์เซนต์ การทดลองครั้งนี้ยืนยันได้ว่า วิธีการเรดิโออิมมูนโนแอสเช ช่วยการตรวจสอบการตั้งครรภ์ในโคนมได้อย่างแม่นยำ และช่วยเพิ่มประสิทธิภาพการสืบพันธุ์ของโคนมได้เป็นอย่างดี
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References
กมลพัฒนะ, มณีวรรณ; ศรีศักดิ์วัฒนะ, กิติยา และ สรรเพชญ, โสภณ. (2531). การตรวจท้องโคนม ระบบบริการ และผลงาน. ใน บทบาทการตรวจท้องโคนมจากน้ำนม. หน้า 73-126. โรงพิมพ์จุฬาลงกรณ์มหาวิทยาลัย, กรุงเทพฯ.
Bulman, C. (1976). Progesterone in milk: a potential aid to dairy management. Span 19 (3): 102-105. Bulman, D.C. (1979). The measurement of progesterone in milk. Br. Vet. J. 135: 460-461.
Booth, J.M., Davies, J. and Holdsworth, R.J. (1979). Use of the milk progesterone test for pregnancy determination. Br. Vet. J. 135: 478-488.
Booth, J.M. and Holdsworth, R.J. (1976). The establishment and operation of a central laboratory for pregnancy testing in cows. Br. Vet. J. 132: 518-528.
Cavestany, D. and Foote, R.H. (1985). The use of milk progestene and electronic vaginal probes as aids in large dairy herd reproductive mangement. Cornell Vet. 75: 441-453.
Dobson, H. (1983). A Radioimmunoassay Laboratory Handbook. Liverpool University Press, Liverpool.
Donaldson, L.E., Bassett, J.M. and Thorburn, G.D. (1970). Peripheral plasma progesterone concentration of cows during puberty, oestrous cycles, pregnancy and lactation, and the effects of undernutrition or exogenous oxytocin on progesterone concentration. J. Endocr. 48: 599-614.
Dobson, H. and Fitzpatrick, R.J. (1976). Clinical application of the progesterone-in-milk test. Br. Vet. J. 132: 538-542.
Darling, J.A.B., Laing, A.H. and Harkness, R.A. (1974). A survey of the steroids in cow's milk. J. Endocr. 62: 291-297.
Eastman, S.A.K. (1979). Methods of improving the accuracy of positive results from milk progesterone pregnancy tests. Br. Vet. J.135: 489-491.
Echternkamp, S.E. (1984). Relationship between LH and cortisol in acutely stressed beef cows. Theriogenology 22: 305-311.
Elmore, R.G. (1986). Using rapid progesterone assay kits to detect open cows. Vet. Med. 81(10): 969-970, 972.
Foulkes, J.A. and Heap, R.B. (1987). Managing fertility in dairy cattle. Span 30(1): 42-44.
Gadsby, J.E., Heap, R.B., Henville, A. and Laing, A.R.C. (1974). A semiautomated technique for the estimation of progesterone in cow's milk and its application to pregnancy diagnosis. Proc. Physiol. Soc., J. Physiol. 242: 3p-5p.
Glencross, R.G., Munro, I.B., Senior, B.E. and Pope, G.S. (1973). Concentrations of oestradiol-17 B, oestrone and progesterone in jugular venous plasma of cows during the oestrous cycle and in early pregnancy. Acta Endocrinologica 73: 374-384.
Ginther, O.J., Nuti, L.C., Garcia, M.C., Wentworth, B.C. and Tyler, W.J. (1976). Factors affecting progesterone concentration in cow's milk and dairy products.J. Anim. Sci. 42: 155-159.
Holdsworth, R.J., Booth, J.M., Sharman, G.A.M. and Rattray, E.A.S. (1979). Radioim-munoassay of progesterone in milk: Development of techniques for large-scale use as a test of pregnancy. Br. Vet. J. 135: 470-477.
Hoffmann, B., Gunzler, O., Hamburger, R. and Schmitt, W. (1976). Milk progesterone as a parameter for fertility control in cattle; methodo-logical approaches and present status of application in Germany. Br. Vet. J. 13: 469-476.
Heap, R.B., Holdsworth, R.J., Gadsby, J.E., Laing, J.A. and Walters, D.E. (1976). Pregnancy diagnosis in the cow from milk progesterone concentration. Br. Vet. J. 132: 445-464.
Hendricks, D.M., Lamond, D.R., Hill, J.R. and Dickey, J.F. (1971). Plasma progesterone concentrations before mating and in early pregnancy in the beef heifer. J. Anim. Sci. 33: 450- 454.
Laitinen, J., Remes, E., Tenhunen, M., Hanninen, O. and Alanko, M. (1985). Milk progesterone in finish dairy cows : A field study on the control of artificial insemination and early pregnancy. Br. Vet. J.141: 297-307.
Macfarlane, J.S., Booth, J.M., Deas, D.W. and Lowman, B.G. (1977). Pregnancy test and evaluation of embryonic and fetal mortality based on progesterone concentration in fore-milk. Vet. Rec. 100: 565-566.
Meredith, S., Kirkpatrick-keller, D. and Butcher, R. (1986). The effects of food restriction and hypophysectomy on numbers of primodial follicles and concentrations of hormones in rats. Biol. Reprod. 35: 68-73.
Nebel, R.L., Whittier, W.D., Cassell, B.G. and Britt, J.M. (1987). Comparison of on-farm and laboratory milk progesterone assays for identifying errors in detection of estrus and diagnosis of pregnancy. J. Dairy Sci. 70: 1471-1476.
Pope, G.S., Majzlik, I., Ball, P.J.H. and Leaver, J.D. (1976). Use of progesterone concentrations in plasma and milk in the diagnosis of pregnancy in domestic cattle. Br. Vet. J. 132: 497-506. Pennington, J.A., Spahr, S.L. and Lodge, J.R. (1976). Factors affecting progesterone in milk for pregnancy diagnosis in dairy cattle. Br. Vet. J. 132: 487-496.
Robbertson, H.A. and Sarda, I.R. (1971). A very early pregnaney test for mammals: Its application to the cow, ewe and sow. J. Endocr. 49: 407-419.
Shemesh, M., Ayalon, N. and Lindner, H.R. (1973). Early pregnancy diagnosis based upon plasma progesterone levels in the cow and ewe. J. Anim. Sci. 36: 726 -729.
Shemesh, M., Ayalon, N., Lavi, S., Mileguir, F., Shore, L.S. and Toby, D. (1983). A new approach to the use of progesterone levels for pregnancy determination. Br. Vet. J: 139: 41-48.
Schiavo, J.J., Matuszczak, R.L., Oltenacu, D.B. and Foote, R.H. (1975). Milk progesterone in postpartum and pregnant cows as a monitor of reproductive status. J. Dairy Sci. 58: 1713-1716.
Stabenfeldt, G.H., Osburn, B.I. and Ewing, L.L. (1970). Peripheral plasma progesterone levels in the cow. Am. J. Physiol. 218: 571-575.
Stanley, C.J., Paris, F., Webb, A.E., Heap, R.B., Ellis, S.T., Hamon, M., Worsfold, A. and Booth, J.M. (1986). Use of a new and rapid milk progesterone assay to monitor reproductive activity in the cow. Vet. Rec. 118: 664-667.
Walters, D.E. (1976). A note on the statistical aspects of pregnancy diagnosis. Br. Vet. J. 132: 465- 468.
Worsfold, A.i., Booth, J.M., Weles, P.W., Huddart, A.C. and Stanley, C.J. (1987). The evaluation of a new rapid milk progesterone test as an aid to improving dairy herd fertility. Br. Vet. J. 143: 83-87.
Wimpy, T.H., Chang, C.F., Estergreen, V.L. and Hillers, J.K. (1986). Milk progesterone enzyme immunoassay: Modifications and a field trial for pregnancy detection in dairy cows. J. Dairy Sci. 69: 1115-1121.
Bulman, C. (1976). Progesterone in milk: a potential aid to dairy management. Span 19 (3): 102-105. Bulman, D.C. (1979). The measurement of progesterone in milk. Br. Vet. J. 135: 460-461.
Booth, J.M., Davies, J. and Holdsworth, R.J. (1979). Use of the milk progesterone test for pregnancy determination. Br. Vet. J. 135: 478-488.
Booth, J.M. and Holdsworth, R.J. (1976). The establishment and operation of a central laboratory for pregnancy testing in cows. Br. Vet. J. 132: 518-528.
Cavestany, D. and Foote, R.H. (1985). The use of milk progestene and electronic vaginal probes as aids in large dairy herd reproductive mangement. Cornell Vet. 75: 441-453.
Dobson, H. (1983). A Radioimmunoassay Laboratory Handbook. Liverpool University Press, Liverpool.
Donaldson, L.E., Bassett, J.M. and Thorburn, G.D. (1970). Peripheral plasma progesterone concentration of cows during puberty, oestrous cycles, pregnancy and lactation, and the effects of undernutrition or exogenous oxytocin on progesterone concentration. J. Endocr. 48: 599-614.
Dobson, H. and Fitzpatrick, R.J. (1976). Clinical application of the progesterone-in-milk test. Br. Vet. J. 132: 538-542.
Darling, J.A.B., Laing, A.H. and Harkness, R.A. (1974). A survey of the steroids in cow's milk. J. Endocr. 62: 291-297.
Eastman, S.A.K. (1979). Methods of improving the accuracy of positive results from milk progesterone pregnancy tests. Br. Vet. J.135: 489-491.
Echternkamp, S.E. (1984). Relationship between LH and cortisol in acutely stressed beef cows. Theriogenology 22: 305-311.
Elmore, R.G. (1986). Using rapid progesterone assay kits to detect open cows. Vet. Med. 81(10): 969-970, 972.
Foulkes, J.A. and Heap, R.B. (1987). Managing fertility in dairy cattle. Span 30(1): 42-44.
Gadsby, J.E., Heap, R.B., Henville, A. and Laing, A.R.C. (1974). A semiautomated technique for the estimation of progesterone in cow's milk and its application to pregnancy diagnosis. Proc. Physiol. Soc., J. Physiol. 242: 3p-5p.
Glencross, R.G., Munro, I.B., Senior, B.E. and Pope, G.S. (1973). Concentrations of oestradiol-17 B, oestrone and progesterone in jugular venous plasma of cows during the oestrous cycle and in early pregnancy. Acta Endocrinologica 73: 374-384.
Ginther, O.J., Nuti, L.C., Garcia, M.C., Wentworth, B.C. and Tyler, W.J. (1976). Factors affecting progesterone concentration in cow's milk and dairy products.J. Anim. Sci. 42: 155-159.
Holdsworth, R.J., Booth, J.M., Sharman, G.A.M. and Rattray, E.A.S. (1979). Radioim-munoassay of progesterone in milk: Development of techniques for large-scale use as a test of pregnancy. Br. Vet. J. 135: 470-477.
Hoffmann, B., Gunzler, O., Hamburger, R. and Schmitt, W. (1976). Milk progesterone as a parameter for fertility control in cattle; methodo-logical approaches and present status of application in Germany. Br. Vet. J. 13: 469-476.
Heap, R.B., Holdsworth, R.J., Gadsby, J.E., Laing, J.A. and Walters, D.E. (1976). Pregnancy diagnosis in the cow from milk progesterone concentration. Br. Vet. J. 132: 445-464.
Hendricks, D.M., Lamond, D.R., Hill, J.R. and Dickey, J.F. (1971). Plasma progesterone concentrations before mating and in early pregnancy in the beef heifer. J. Anim. Sci. 33: 450- 454.
Laitinen, J., Remes, E., Tenhunen, M., Hanninen, O. and Alanko, M. (1985). Milk progesterone in finish dairy cows : A field study on the control of artificial insemination and early pregnancy. Br. Vet. J.141: 297-307.
Macfarlane, J.S., Booth, J.M., Deas, D.W. and Lowman, B.G. (1977). Pregnancy test and evaluation of embryonic and fetal mortality based on progesterone concentration in fore-milk. Vet. Rec. 100: 565-566.
Meredith, S., Kirkpatrick-keller, D. and Butcher, R. (1986). The effects of food restriction and hypophysectomy on numbers of primodial follicles and concentrations of hormones in rats. Biol. Reprod. 35: 68-73.
Nebel, R.L., Whittier, W.D., Cassell, B.G. and Britt, J.M. (1987). Comparison of on-farm and laboratory milk progesterone assays for identifying errors in detection of estrus and diagnosis of pregnancy. J. Dairy Sci. 70: 1471-1476.
Pope, G.S., Majzlik, I., Ball, P.J.H. and Leaver, J.D. (1976). Use of progesterone concentrations in plasma and milk in the diagnosis of pregnancy in domestic cattle. Br. Vet. J. 132: 497-506. Pennington, J.A., Spahr, S.L. and Lodge, J.R. (1976). Factors affecting progesterone in milk for pregnancy diagnosis in dairy cattle. Br. Vet. J. 132: 487-496.
Robbertson, H.A. and Sarda, I.R. (1971). A very early pregnaney test for mammals: Its application to the cow, ewe and sow. J. Endocr. 49: 407-419.
Shemesh, M., Ayalon, N. and Lindner, H.R. (1973). Early pregnancy diagnosis based upon plasma progesterone levels in the cow and ewe. J. Anim. Sci. 36: 726 -729.
Shemesh, M., Ayalon, N., Lavi, S., Mileguir, F., Shore, L.S. and Toby, D. (1983). A new approach to the use of progesterone levels for pregnancy determination. Br. Vet. J: 139: 41-48.
Schiavo, J.J., Matuszczak, R.L., Oltenacu, D.B. and Foote, R.H. (1975). Milk progesterone in postpartum and pregnant cows as a monitor of reproductive status. J. Dairy Sci. 58: 1713-1716.
Stabenfeldt, G.H., Osburn, B.I. and Ewing, L.L. (1970). Peripheral plasma progesterone levels in the cow. Am. J. Physiol. 218: 571-575.
Stanley, C.J., Paris, F., Webb, A.E., Heap, R.B., Ellis, S.T., Hamon, M., Worsfold, A. and Booth, J.M. (1986). Use of a new and rapid milk progesterone assay to monitor reproductive activity in the cow. Vet. Rec. 118: 664-667.
Walters, D.E. (1976). A note on the statistical aspects of pregnancy diagnosis. Br. Vet. J. 132: 465- 468.
Worsfold, A.i., Booth, J.M., Weles, P.W., Huddart, A.C. and Stanley, C.J. (1987). The evaluation of a new rapid milk progesterone test as an aid to improving dairy herd fertility. Br. Vet. J. 143: 83-87.
Wimpy, T.H., Chang, C.F., Estergreen, V.L. and Hillers, J.K. (1986). Milk progesterone enzyme immunoassay: Modifications and a field trial for pregnancy detection in dairy cows. J. Dairy Sci. 69: 1115-1121.