Effect of Agitation and Aeration on the Growth of Aurantiochytrium sp. FIKU018 Fed-batch Cultured in Bioreactor ผลของอัตราการกวนและการให้อากาศต่อการเจริญเติบโตของ Aurantiochytrium sp. FIKU018 ที่เพาะเลี้ยงแบบเฟด-แบทช์ ในถังปฏิกรณ์ชีวภาพ

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Katsaya Khumrangsee
Theppanya Charoenrat
Natinee Suvanasingha
Supenya Chittapun

Abstract

Agitation and aeration are important factors, which play a vital role in cell characteristics and cell growth during cultivation in the bioreactor. This research aimed to study the effects of agitation and aeration rates on the growth of Aurantiochytrium sp. FIKU018 cultured in the fermenter. The fed-batch cultivation of Aurantiochytrium sp. FIKU018 was conducted in a 22 L bioreactor, feeding with GYP medium in batch phase and with GYP feed and glucose feed during the fed-batch stage. The process was operated at 30°C, pH 6.0 under different agitation (450-800 rpm) and aeration (0-1.5 vvm) rates. The sample was collected every 4 h to measure the absorbance, determine dry cell weight and analyze the concentration of reducing sugar. The results showed that the stirring rate of 450 rpm and associated with an appropriate aeration rate, which adjusted according to the cell growth was proper to enabled the fed-batch cell culture to continue for 64 h with the specific growth rate of 0.21 h-1 and the maximum dry cell weight of 30.50±0.44 g/L. The yield at the end of the culture in the fed-batch system was 0.45±0.05 g cell/g glucose. This finding can be used as fundamental data to develop Aurantiochytrium sp. FIKU018 cultivation in the bioreactor for further upscale to the industrial level.

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