Total Phenolic Compounds and Cytotoxicity in Brine Shrimp and Aedes aegypti Larvae of Thurian-thet (Annona muricata L.) Crude Extracts
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Abstract
This research was conducted to determine total phenolic compounds and assess cytotoxicity in brine shrimp and Aedes aegypti larvae of crude extracts from various parts of Thurian-thet: unripe fruit, ripe fruit, leaves, seeds and stem bark achieved by adopting different solvents which are hexane, dichloromethane, ethanol, and methane. The maximum phenolic content was found in ethanolic ripe fruit extract at 2,036±5.99 microgram of gallic acid equivalent per milligram of extract (µg GAE/g ext). The crude extracts at concentrations of 10-1,000 microgram per milliliter (µg/mL) were tested for cytotoxicity in brine shrimp and Aedes aegypti larvae. It was found that the lowest lethal concentration fifty (LC50) tested in brine shrimp was 71.95 µg/mL (24 h) from methanolic ripe fruit extract and in mosquito larvae was 21.39 µg/mL (24 h) from ethyl alcoholic ripe fruit extract. The results suggested that the ethanolic ripe fruit extract contained the maximal total phenolic compounds which could be used as natural antioxidants. The ethanolic ripe fruit extract could also be used as a mosquito natural repellent since after being tested with mosquito larva and found the lowest value of LC50. However, the toxicity tests of Thurian-thet ripe fruit extract in laboratory animals should be determined in the future for possible applications in food and medicine.
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เนื้อหาและข้อมูลในบทความที่ลงตีพิมพ์ในวารสารวิชชา มหาวิทยาลัยราชภัฏนครศรีธรรมราช ถือเป็นข้อคิดเห็นและความรับผิดชอบของผู้เขียนบทความโดยตรง ซึ่งกองบรรณาธิการวารสารไม่จำเป็นต้องเห็นด้วยหรือร่วมรับผิดชอบใด ๆ
บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการตีพิมพ์ในวารสารวิชชา มหาวิทยาลัยราชภัฏนครศรีธรรมราช ถือเป็นลิขสิทธ์ของวารสารวิชชา มหาวิทยาลัยราชภัฏนครศรีธรรมราช หากบุคคลหรือหน่วยงานใดต้องการนำข้อมูลทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือเพื่อการกระทำการใด ๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษรจากวารสารวิชชา มหาวิทยาลัยราชภัฏนครศรีธรรมราชก่อนเท่านั้น
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