การเจริญเติบโตของข้าวสังข์หยดพัทลุงที่ได้จากการเพาะเลี้ยงเนื้อเยื่อพืชรุ่นที่ 2 ในสภาพโรงเรือนพลาสติก
Article Sidebar
Main Article Content
บทคัดย่อ
Sang Yod Phatthalung rice has dark-red color seeds and a high antioxidant activity. The current desire of healthy food leads to the high demand of healthier rice product. Thus, investigation of PGRs on vegetative and reproductive growth after transferring to plastic house conditions at Faculty of Natural Resources, Prince of Songkla University, Hat Yai campus was conducted. The original and first somaclonal seeds were obtained from fist regeneration (SC1R1 seeds) in different plant growth regulators (PGRs) containing liquid oil palm culture medium (OPCM) which were sown in vitro. Shoot tips were excised and cultured in liquid OPCM without and with different concentrations of 6-benzyladenine (BA) (0, 0.5, 1.0 and 1.5 milligram per liter (mg/L) alone or in combination with 0.5 mg/L 1-naphthaleneacetic acid (NAA). Complete plantlets (SC1R2 plants) were acclimatized and transferred to plastic house conditions. After acclimatizing for 80 and 140 days, vegetative and reproductive growth characteristics were recorded and statistically compared among the above treatments (different PGRs) using completely randomized design (CRD). The results revealed that plants obtained from OPCM medium supplemented with 0.5 mg/L BA containing the medium (SCV1R2+0.5BA) gave the highest vegetative growth of leaf length at 68.50 centimeter (cm), leaf width at 1.08 cm, stem diameter at 0.93 cm and plant height at 186.50 cm. For reproductive growth, the similar result was obtained. BA at concentration of 0.5 mg/L containing the medium (SC1R2+0.5BA) provided the shortest time for 1st flowering at 97 days, 50% flowering at 99 days, the highest number of panicles per plant at 40 panicles and 100 grain weight at 1.566 gram (g). From the results, the acquired knowledge can be applied to improve vegetative growth, reproductive growth, and higher productivity for further success.
Article Details
อนุญาตภายใต้เงื่อนไข Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
เนื้อหาและข้อมูลในบทความที่ลงตีพิมพ์ในวารสารวิชชา มหาวิทยาลัยราชภัฏนครศรีธรรมราช ถือเป็นข้อคิดเห็นและความรับผิดชอบของผู้เขียนบทความโดยตรง ซึ่งกองบรรณาธิการวารสารไม่จำเป็นต้องเห็นด้วยหรือร่วมรับผิดชอบใด ๆ
บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการตีพิมพ์ในวารสารวิชชา มหาวิทยาลัยราชภัฏนครศรีธรรมราช ถือเป็นลิขสิทธ์ของวารสารวิชชา มหาวิทยาลัยราชภัฏนครศรีธรรมราช หากบุคคลหรือหน่วยงานใดต้องการนำข้อมูลทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือเพื่อการกระทำการใด ๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษรจากวารสารวิชชา มหาวิทยาลัยราชภัฏนครศรีธรรมราชก่อนเท่านั้น
The content and information in the article published in Wichcha journal Nakhon Si Thammarat Rajabhat University, It is the opinion and responsibility of the author of the article. The editorial journals do not need to agree. Or share any responsibility.
เอกสารอ้างอิง
Amin, A.A., Rashad, E.S.M., Hassanein, M.S. and Zaki, N.M. (2007). Response of some white maize hybrids to foliar spray with benzyl adenine. Research Journal of Agriculture and Biological Sciences, 3(6), 648-656.
Ananiev, E.D., Ananieva, K., Todorov, I., Popov, M. and Bonchev, G. (2004). Effect of methyl ester of jasmonic acid, abscisic acid and benzyladenine on chlorophyll synthesis in excised cotyledons of Cucurbita pepo (Zucchini). Bulgarian Journal of Plant Physiology, 30(1), 51-63.
Chuenboonngarm, N., Charoonsotea, S. and Bhamarapravatia, S. (2001). Effect of BA and 2iP on shoot proliferation and somaclonal variation of Gardenia jasminoides Ellis in vitro culture. ScienceAsia, 27(1), 137-141.
Cioc, M., Kalisz, A., Zupnik, M. and Pawlowska, B. (2019). Different LED light intensities and 6-benzyladenine concentrations in relation to shoot development, leaf architecture, and photosynthetic pigments of Gerbera jamesonii Bolus in vitro. Agronomy, 9(7), doi: https://doi.org/10.3390/agronomy9070358.
Dobranszki, J. and Mendler-Drienyovszki, N. (2014). Cytokinin-induced changes in the chlorophyll content and fluorescence of in vitro apple leaves. Journal of Plant Physiology, 171(16), 1472-1478, doi: https://doi.org/10.1016/j.jplph.2014.06.015.
Emaldi, U., Trujillo, I. and Gracia, E.D. (2004). Comparison of characteristics of bananas (Musa sp.) from the somaclone CIEN BTA-03 and its parental clone Williams. Fruit, 59(4), 257-263, doi: https://doi.org/10.1051/fruits:2004024.
Gao, Z., Liang, X.G., Zhang, L., Lin, S., Zhao, X., Zhou, L.L., Shen, S. and Zhou, S.L. (2017). Spraying exogenous 6-benzyladenine and brassinolide at tasseling increases maize yield by enhancing source and sink capacity. Field Crops Research, 211, doi: https://doi.org/10.1016/j.fcr.2017.05.027.
Gupta, N.K., Gupta, S., Shukla, D.S., Deshmukh, P.S. (2003). Differential responses of BA injection in maize temperate on yield and specific grain growth in contrasting genotypes of wheat (Triticum aestivum L.). Journal of Plant Growth Regulation, 40(3), 201-205, doi: https://doi.org/10.1023/A:1025023822806.
Hazrati, S., Sarvestani, Z.T. and Babaei, A. (2011). Enhancing yield and aloin concentration of Aloe vera plants by simultaneous application of N and benzyladenine. Journal of Medicinal Plants Research, 6(10), 1834-1841, doi: https://doi.org/10.5897/JMPR11.499.
Ho, T.L., Te-Chato, S. and Yenchon, S. (2018). Callus induction and plantlet regeneration systems in Indica rice (Oryza sativa L.) cultivar Sangyod. Walailak Journal of Science and Technology, 15(10), 753-763, doi: https://doi.org/10.48048/wjst.2018.3772.
Hosseini, S.M., Poustini, K. and Ahmadi, A. (2008). Effects of foliar application of BAP on source and sink strength in four six-rowed barley (Hordeum vulgare L.) cultivars. Journal of Plant Growth Regulation, 54(3), 231-239, doi: https://doi.org/10.1007/s10725-007-9245-4.
Joshi, R.K. and Rao, G.J.N. (2009). Somaclonal variation in submergence tolerant rice cultivars and induced diversity evaluation by PCR markers. International Journal of Genetics and Molecular Biology, 1(5), 80-88.
Karp, A. (1995). Somaclonal variation as a tool for crop improvement. Euphytica, 85, 295-302, doi: https://doi.org/10.1007/BF00023959.
Kumar, N. and Reddy, M.P. (2011). In vitro plant propagation: A review. Journal of Forest Science, 27(2), 61-72.
Leshem, B., Ronen, R., Soudry, E., Lurie, S. and Gepstein, S. (1994). Cytokinin at a large range of concentrations determines rates of polypeptide metabolism in cultured melon cotyledons. Journal of Plant Physiology, 143(3), 330-336, doi: https://doi.org/10.1016/S0176-1617(11)81640-8.
Leva, A.R., Petruccelli, R. and Rinaldi, L.M.R. (2012). Somaclonal variation in tissue culture: A case study with olive. Recent Advances in Plant In Vitro Culture, 1(1), 123-150, doi: http://dx.doi.org/10.5772/50367.
Marandi, J., Naseri, R., Mohseniazer, L., Hajitagiloo, M. and Marhamati, M.R. (2011). Investigation on interaction effect of benzyladenine and chitosan on in vitro proliferation of strawberry (Fragaria x ananassa cv. Selva). Agricutural Biotechnology, 10(1), 27-34.
Mehta, Y.R. and Angra, D.C. (2000). Somaclonal variation for disease resistance in wheat and production of dihaploids through wheat 9 maize hybrids. Genetics and Molecular Biology, 23(3), 617-622, doi: https://doi.org/10.1590/S1415-47572000000300021.
Mustafa, I.S. and Taha, R.A. (2012). Influence of plant growth regulators and subculturing on in vitro multiplication of some fig (Ficus carica) cultivars. Journal of Applied Sciences Research, 8(8), 4038-4044.
Noimusik, P., Te-chato, S. and Yenchon, S. (2018). Effect of plant growth regulators on shoot multiplication and root induction from culturing shoot tips of Sang Yod Muang Phatthalung rice. In the 11th IMT-GT UNINET Conference 2018- Bioscience for A Sustainable Future (pp. 130-136). Penang: Malaysia.
Orbovic, V., Calovic, M., Viloria, Z., Nielsen, B., Gmitter, F.G., Castle, W.S. and Grosser, J.W. (2008). Analysis of genetic variability in various tissue culture-derived lemon plant populations using RAPD and flow cytometry. Euphytica, 161(3), 329-335, doi: https://doi.org/10.1007/s10681-007-9559-3.
Patnaik, J., Sahoo, S. and Debata, B.K. (1999). Somaclonal variation in cell suspension culture-derived regenerants of Cymbopogon martinii (Roxb.) Wats var. motia. Plant Breeding, 118(4), 351-354, doi: https://doi.org/10.1046/j.1439-0523.1999.00383.x.
Predieri, S. (2001). Mutation induction and tissue culture in improving fruits. Plant Cell, Tissue and Organ Culture, 64(2), 185-210, doi: https://doi.org/10.1023/A:10106 23203554.
Rice Department. (2010). Sang Yod Phatthalung rice, first cultivar obtained geographical indications (GI) in Thailand. Bangkok: Rice Product Development Division, Rice Department.
Rueb, S., Leneman, M., Schilperoort, R.A. and Hensgens, L.A.M. (1994). Efficient lant regeneration through somatic embryogenesis from callus induced on mature rice embryos (Oryza sativa L.). Plant Cell, Tissue and Organ Culture, 36(2), 259-264, doi: https://doi.org/10.1007/BF00037729.
Sardoei, A.S., Rahbarian P. and Shahdadneghad, M. (2014). Evaluation chlorophyll contents assessment on three indoor ornamental plants with plant growth regulators. European Journal of Experimental Biology, 4(2), 306-310.
Skirvin, R.M., McPheeters, K.D., Norton, M. and Veilleux, R. (1994). Sources and frequency of somaclonal variation. Horticultural Science, 29(11), 1232-1237, doi: https://doi.org/10.21273/HORTSCI.29.11.1232.
Song, J. (2003). Rice science: Innovations and impact for livelihood. In proceedings of the International Rice Research Conference (pp. 5-7). Beijing: International Rice Research Institute, Chinese Academy of Engineering, and Chinese Academy of Agricultural Sciences.
Wang, Y., Lu, J.W., Ren, T., Li, P.F., Liu, Q.X. and Li, X.K. (2020). Effects of exogenous cytokinin on photosynthesis, senescence and yield performance of inferior rice tillers grown under different nitrogen regimes. Photosynthetica, 58(1), 137-145, doi: https://doi.org/10.32615/ps.2019.170.
Yeh, S.Y., Chen, H.W., Ng, C.Y., Lin, C.Y., Tseng, T.H., Li, W.H. and Ku, M.S.B. (2015). Down-regulation of cytokinin oxidase 2 expression increases tiller number and improves rice yield. Rice, 8(36), 2-13, doi: https://doi.org/10.1186/s12284-015-0070-5.
Yinxia, Z. and Te-chato, S. (2013). Improved plantlet regeneration systems in Indica rice (Oryza sativa L.) landrace Hom Kra Dang Ngah. Journal of Agricultural Technology, 9(6), 1641-1654.
Yodmanee, S., Karrila, T.T. and Pakdeechanuan, P. (2011). Physical, chemical and antioxidant properties of pigmented rice grown in Southern Thailand. International Food Research Journal, 18(3), 901-906.
Zheng, C.F., Zhu, Y.J., Wang, C.Y. and Guo, T.C. (2016). Wheat grain yield increase in response to pre-anthesis foliar application of 6-benzylaminopurine is dependent on floret development. Plos One, 11(6), doi: https://doi.org/10.1371/journal.pone.0156627.
