Effect of Heat Shock on Viability of Oviductal Epithelial Cell and Porcine Oocytes

Abstract

The experiments aimed to study viability rate of oviduct epithelial cell (OEC) and oocytes after exposed heat shock (EHS) in vitro culture. Study 1, to study on the viability rate of OEC after EHS different temperatures. Oviducts were collected from ovaries 18 gilts slaughterhouse. Completely randomized design was used. There were 6 groups (group 1 EHS 38.5°C 12 h, group 2 EHS 38.5°C 24 h, group 3 EHS 40°C 12 h, group 4 EHS 40°C 24 h, group 5 EHS 42°C 12 h and group 6 EHS 42°C 24 h). The results showed that group 1 (99.09±0.68%) had viability rate than group 4 (97.45±1.43%), group 5 (95.90±2.29%) and group 6 (95.88±1.89%) with statistical significance (P<0.01) but not different from with group 2 (98.95±0.56%), group 3 (97.59±1.31%) (P<0.01). Study 2, study on cumulus-oocyte complexes (COCs) co-culture OEC, EHS with the different temperature was determined in vitro culture system. Ovaries were collected from 300 gilts. The results showed that after culture for 44 h in group 1 (6.23±0.90 µm) was expanded of cumulus cells higher than group 3 (5.86±0.88 µm), group 4 (5.85± 0.90 µm), group 5 (5.86±0.95 µm) and group 6 (5.70±1.09 µm) with statistical significance (P<0.01) but not significance with group 2 (6.21±0.93 µm) (P>0.01). Development rate of oocytes to metaphase II stage in group 1 (67.21%) was higher than group 2 (66.13%), group 3 (62.40%), group 4 (61.81%), group 5 (62.10%) and group 6 (61.82%). It was concluded that high temperature decreases viability rate of OEC, expanded of cumulus cells COCs and development rate of oocytes to metaphase II stage.