Optimum Conditions for Indole-3-Acetic Acid Production by Streptomyces and its Stimulation on Seed Germination of Rice cv. KDML105

Authors

  • Khanitta Somtrakoon Department of Biology, Faculty of Science, Mahasarakham University, Kantharawichai, Mahasarakham
  • Nattamon Sripasa Department of Biology, Faculty of Science, Mahasarakham University, Kantharawichai, Mahasarakham
  • Sudarat Ladsena Department of Biology, Faculty of Science, Mahasarakham University, Kantharawichai, Mahasarakham
  • Aphidech Sangdee Department of Biology, Faculty of Science, Mahasarakham University, Kantharawichai, Mahasarakham
  • Waraporn Chouychai Biology Program, Faculty of Science and Technology, Nakhonsawan Rajabhat University, Nakhonsawan

Keywords:

indole-3-acetic acid, rice KDML105,, Streptomyces

Abstract

This study was to investigate the effects of temperature, pH and NaCl on indole-3-acetic acid produced by 4 bacterial species isolated from vermicompost, that were Streptomyces sp. SEF4, Streptomyces sp. SEF11, Streptomyces sp. SEF28 and Streptomyces sp. SEF37. Effect of the culture filtrate from four strains of Streptomyces on germination stimulated and seedling growth of rice cultivar KDML105 were also determined. The results showed that the optimum of indole-3-acetice acid produced by four strains of Streptomyces were observed at 27-30ºC (18.8-20.7 mg/l) and pH 7-9. Streptomyces sp. SEF4, Streptomyces sp. SEF11, Streptomyces sp. SEF28 could produce indole-3-acetic acid in the presence of 2.55% NaCl. However, using suspension of these bacterial isolates could not stimulate seed germination and seedling growth of rice cultivar KDML105. The culture filtrate of Streptomyces sp. SEF11, Streptomyces sp. SEF28 and Streptomyces sp. SEF37 trended to stimulate germination of seed while culture filtrate of Streptomyces sp. The SEF4 and Streptomyces sp. SEF11 trended to increase shoot length but culture filtrate of all strains decreased the root length. Thus, these Streptomyces strains could produce indole-3-acetic acid under alkaline conditions and salt stress conditions which could be applied to use with rice cultivation in the North eastern of Thailand in the future.

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Published

2019-12-28

How to Cite

Somtrakoon, K. ., Sripasa, N. ., Ladsena, S. ., Sangdee, A. ., & Chouychai, W. . (2019). Optimum Conditions for Indole-3-Acetic Acid Production by Streptomyces and its Stimulation on Seed Germination of Rice cv. KDML105. Journal of Agricultural Research and Extension, 36(3), 12–22. retrieved from https://li01.tci-thaijo.org/index.php/MJUJN/article/view/232882

Issue

Vol. 36 No. 3 (2019): September - December 2019

Section

Research Article

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