Study of the Use of Chemicals to Reduce Microbial Contamination and Its Effect on the Growth of Sugarcane Tissue in an Open System
DOI:
https://doi.org/10.14456/jare-mju.2025.6Keywords:
sugarcane, antimicrobial substances, low-cost tissue culture, open tissue cultureAbstract
This study aims to determine the type and concentration of disinfectants that inhibit microbial growth in laboratory conditions while being suitable for sugarcane tissue culture. In the initial step, microorganisms contaminating the Murashige and Skoog (MS) in an open laboratory system (November 2020) were screened. Eight fungal isolates were found, including Aspergillus sp., Trichoderma sp., Fusarium sp., Gliocladium sp., Curvularia sp. and a yeast, as well as two unknown species lacking identifiable fruiting structures. Five bacterial isolates were also identified, consisting of one gram-positive and four gram-negative bacteria. Subsequently, the type and concentration of disinfectants were tested to control each screened microbial contaminant in the MS medium. It was found that sodium hypochlorite at concentrations of 0.17, 0.25, and 0.33 ml/L effectively controlled microbial contamination. In the sugarcane tissue culture experiment using non-autoclaved MS medium supplemented with 1 mg/L kinetin, 150 mg/L citric acid, and sodium hypochlorite at concentrations of 0.20, 0.25, and 0.33 ml/L, good growth and development of sugarcane tissue were observed, with no significant differences compared to traditional tissue culture methods.
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