Comparison of Stirring Patterns Suitable for the Growth of Chlorella sp.

Authors

  • Kanyarat Pooltajit Department Aquacultural Technology and Aquatic Resource Management, King Mongkut's Institute of Technology Ladkrabang Bangkok
  • Nongnuch Laohavisuti Department Aquacultural Technology and Aquatic Resource Management, King Mongkut's Institute of Technology Ladkrabang Bangkok
  • Somkiat Seesanong Office of Administrative Interdisciplinary Program on Agricultural Technology, Faculty of Agricultural Technology King Mongkut's Institute of Technology Ladkrabang, Bangkok
  • Uscharee Ruangdej Department Aquacultural Technology and Aquatic Resource Management, King Mongkut's Institute of Technology Ladkrabang Bangkok

DOI:

https://doi.org/10.14456/jare-mju.2025.3

Keywords:

Chlorella sp., growth, submersible pump, stirring

Abstract

The study aimed to compare suitable stirring patterns for the growth of Chlorella sp.  A completely randomized experimental design was conducted with four sets of experiments, each repeated five times. Set 1 served as the control with air-stone bubbler stirring. Sets 2 and 3 utilized horizontal and vertical stirring circulation, respectively, while Set 4 combined horizontal and vertical stirring circulation. Chlorella sp. cells, initially at 9.8×104 cells/mL, were cultured outdoors for 10 days using modified nutrient media. Growth parameters, including cell count, dry weight, chlorophyll a content, pheophytin content, and pH were recorded. The results showed that all experimental sets reached maximum growth rates on day 8, with values of 789.64×104, 721.63×104, 1,116.28×104 and 1,580.08×104 cells/mL, respectively, significantly differing (P<0.05). Dry weight values were 123.60, 177.60, 164.00 and 202.80 µg/mL, respectively, also significantly different (P<0.05). Chlorophyll a content was 3,859.81, 4,756.69, 4,068.30 and 5,741.60 µg/L, respectively (P<0.05). Pheophytin content did not significantly differ (P>0.05), and pH values showed minor alkalinity, favoring Chlorella sp. growth. In conclusion, the combined horizontal and vertical water circulation system proved to be the most suitable stirring pattern for cultivating Chlorella sp., resulting in superior cell count, dry weight, chlorophyll a content, and pheophytin content compared to other experimental sets.

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Published

2025-04-25

How to Cite

Pooltajit, K., Laohavisuti, N., Seesanong, S., & Ruangdej, U. . (2025). Comparison of Stirring Patterns Suitable for the Growth of Chlorella sp. Journal of Agricultural Research and Extension, 42(1), 82–93. https://doi.org/10.14456/jare-mju.2025.3

Issue

Vol. 42 No. 1 (2025): January-April 2025

Section

Research Article

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