การศึกษาเปรียบเทียบปริมาณสารออกฤทธิ์ทางชีวภาพและสภาวะที่เหมาะสมในการสกัดสาร โดยเทคนิคไมโครเวฟในดอกกล้วยไม้เอื้องผึ้ง

Jutamas  Piladee; Narin  Taokaenchan; Watcharaporn  Sukkhee; Geeranan  Takam; Boontan  Suthep; Sureechon  Wongparsit

doi:10.14456/jare-mju.2026.10

Authors

Jutamas Piladee Horticulture, Faculty of Agricultural Production, Maejo University, Chiang Mai, Thailand https://orcid.org/0009-0009-5845-2863
Narin Taokaenchan Medicinal Plant Science, Faculty of Agricultural Production, Maejo University, Chiang Mai, Thailand
Watcharaporn Sukkhee Special Affairs Department, Faculty of Agricultural Production, Maejo University, Chiang Mai, Thailand
Geeranan Takam Special Affairs Department, Faculty of Agricultural Production, Maejo University, Chiang Mai, Thailand
Boontan Suthep Special Affairs Department, Faculty of Agricultural Production, Maejo University, Chiang Mai, Thailand
Sureechon Wongparsit Special Affairs Department, Faculty of Agricultural Production, Maejo University, Chiang Mai, Thailand

DOI:

https://doi.org/10.14456/jare-mju.2026.10

Keywords:

Dendrobium lindleyi Steud, bioactive compounds, flavonoid

Abstract

This study aims to compare the amount of bioactive compounds and determine the optimal extraction conditions using microwave-assisted extraction in the flower of Dendrobium Lindleyi Steud. Comparative tests were conducted on the amounts of substances extracted from the stem, leaf, flower, and root parts using the microwave technique. The obtained extracts were analyzed for total phenolic content using the Folin–Ciocalteu method, total flavonoid content using the aluminum chloride colorimetric method, antioxidant activity via the DPPH radical scavenging assay, and tyrosinase inhibition activity using the modified dopachrome method. Results indicated that the flower contained the highest amount of flavonoids at 32.53±0.76 mg QE g^-1 DW. Therefore, further experiments were conducted to optimize the microwave extraction conditions by varying several factors, including microwave power, extraction time, ethanol-to-water ratio, and number of extraction cycles. The optimal conditions were found to be 600 watts of microwave power, 270 seconds of extraction time, an ethanol-to-water ratio of 100:0, and one extraction cycle. This study demonstrates that the flower of D. lindleyi Steud. contains bioactive compounds with potential for use in medicinal or skincare products. Moreover, understanding the appropriate microwave extraction conditions provided a guideline for researchers, students, and interested individuals.

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