Development of Grey Hair Coverage Mild Shampoo from Herbal Extracts with Oryza sativa L. and Sesamum indicum L. Extracts

Authors

  • Sanipon Chanburee Division of Health and Cosmetic Product Development, Faculty of Food and Agricultural Technology Pibulsongkram Rajabhat University, Phitsanulok
  • Pruchayachai Chamchoi Division of Law, Faculty of Social Science and Local Development, Pibulsongkram Rajabhat University Phitsanulok
  • Yanisa Chindaloung Division of Health and Cosmetic Product Development, Faculty of Food and Agricultural Technology Pibulsongkram Rajabhat University, Phitsanulok
  • Thanapon Kitpot Division of Health and Cosmetic Product Development, Faculty of Food and Agricultural Technology Pibulsongkram Rajabhat University, Phitsanulok

DOI:

https://doi.org/10.14456/jare-mju.2025.34

Keywords:

Grey hair coverage shampoo, Herbal hair dye, Herbal extracts, Total phenolic, Total Flavonoid

Abstract

Most of hair coloring products normally contain synthetic chemicals which can cause irritation, skin allergy and carcinogen. The aim of this study was to develop a grey hair coverage shampoo from using Oryza sativa L. and Sesamum indicum L. extracts as active ingredients together with Lawsonia inermis L. and Indigofera tinctoria L. powder as natural colorants. The total phenolic contents of Oryza sativa L. and Sesamum indicum L. were 328.02±5.06 and 210.40±6.19 mg GAE/100g extracts, respectively.   The total flavonoid contents were 69.17±0.94 and 50.60±0.22 mg CE/100g extracts, respectively.          The grey hair coverage shampoo was developed using 1% of Oryza sativa L. and Sesamum indicum L. extracts and 2% of Lawsonia inermis L. and Indigofera tinctoria L. powder. This shampoo product was formulated for the elderly and those who have sensitive skin as it was chemical free (not containing phenylenediamine, hydrogen peroxide, ammonia, sodium lauryl sulfate, parabens and silicone). The product had a black color, no pungent smell and was slightly alkali (pH 8). No phase separation was observed from the stability test. This shampoo was easy to use, did not reguire mixing and could cover gray hair for 14 days. The assessors gave a score of 7.6±0.9 points from a 9-point scores of sensory evaluation test.

References

Borrás-Linares, I., S. Fernández-Arroyo, D. Arráez-Roman, P.A. Palmeros-Suárez, R. Del Val-Díaz, I. Andrade-Gonzáles and A. Fernández-Gutiérrez, J.F. Gómez-Leyva, A. Segura-Carretero. 2015. Characterization of phenolic compounds, anthocyanidin, antioxidant and antimicrobial activity of 25 varieties of Mexican Roselle (Hibiscus sabdariffa). Industrial Crops and Products 69: 385–394.

Chaipradit, K., S. Preeprame and S. Naulkaew. 2012. A comparative study of blue pea extract gel and CRC extract gel in the hair pieces of gray-hair. Isan Journal of Pharmaceutical Sciences 8(1): 215–220. [in Thai]

Chompreeda, P. 2007. Sensory Evaluation and Consumer Acceptance. Bangkok: Kasetsart University. 265 p. [in Thai]

Kaikeaw, M. 2009. Development of Hair Dye Spray from Sappan Wood. Master Thesis. Mae Fah Luang University. 51 p. [in Thai]

Manosroi, A. and J. Manosroi. 2013. Cosmeceuticals. Bangkok: Odient Store Publishing. 495 p. [in Thai]

Mokrzycki, W.S. and M. Tatol. 2011. Color difference ∆E - a survey. Machine Graphics and Vision 20(4): 383–411.

Plangmal, K., P. Leelapornpisol, C. Ruengorn and W. Poomanee. 2023. Knowledge and behaviors on hair dying among the students of Rajabhat Mahasarakham University. Thai Journal of Pharmacy Practice 15(2): 491–500. [in Thai]

Raksaphort, S., K. Yenyaem, S. Gluntang, W. Champati, P. Tammakeson, S. Ngogngam and S. Suwancharoen. 2021. Parameters investigation of hair dyeing using natural extract from henna leaves, sappan heartwood, butterfly pea flowers, mangosteen husk and red cabbage. Rajamangala University of Technology Srivijaya Research Journal 13(3): 790–803. [in Thai]

Saithong, A. 2012. Natural dyed indigo silk. KKU Science Journal 40(2): 423–435. [in Thai]

Somrug, K., P. Timsuksai and S. Jenweerawat. 2020. Influence of harvesting date on yield and seed quality of indigo (Indigofera tinctoria L.). King Mongkut's Agricutural Journal 38(4): 426–433. [in Thai]

Sudtim, K., K. Chaijinda and A. Wongrathanachai. 2018. A Survey of hair dye products in beauty salon that Comple with the law: a case study Taling Chan district, Bangkok. Thai Food and Drug Journal 22(1): 51–60. [in Thai]

Sukakul, T., P. Limphoka and W. Boonchai. 2018. Hair dye allergy and hair dyes in Thai market: a literature review and recommendations. Thai Journal of Dermatology 34(4): 245–265. [in Thai]

Sun, M., Y. Deng, X. Cao, L. Xiao, Q. Ding, F. Luo, P. Huang, Y. Gao, M. Liu and H. Zhao. 2022. Effects of natural polyphenols on skin and hair health: a review. Molecules 27(22): 7832.

Thitipramote, N., S. Imsonpang, P. Sukphopetch, P. Pradmeeteekul, J. Nimkamnerd, W. Nantitanon and W. Chaiyana. 2022. Health benefits and safety of red pigmented rice (Oryza sativa L.): in vitro, cellular, and in vivo activities for hair growth promoting treatment. Cosmetics 9(6): 111.

Trüeb, R.M. 2009. Oxidative stress in ageing of hair. International Journal of Trichology 1(1): 6–14.

Trüeb, R.M. 2015. The impact of oxidative stress on hair. International Journal of Cosmetic Science 37(Suppl 2): 25–30.

Weerapreeyakul, N., N. Chansri, A. Lawong and S. Waithong. 2013. Satisfaction study of the application of black sesame meal scrub cream. Isan Journal of Pharmaceutical Sciences 9(1): 222–226. [in Thai]

Yartchimplee, A., P. Khumphai, N. Sakkayawong and C. Sompark. 2021. Antioxidant activities of the indigo extracts and prediction of tyrosinase inhibition by molecular docking. Thai Journal of Science and Technology 10(4): 469–486. [in Thai]

Zhang, Y., C. Kim and T. Zheng. 2012. Hair dye use and risk of human cancer. Frontiers in Bioscience (Elite edition) 4(1): 516–528.

Published

2025-08-26

How to Cite

Chanburee, S. ., Chamchoi, P., Chindaloung, Y., & Kitpot, T. (2025). Development of Grey Hair Coverage Mild Shampoo from Herbal Extracts with Oryza sativa L. and Sesamum indicum L. Extracts. Journal of Agricultural Research and Extension, 42(2), 161–174. https://doi.org/10.14456/jare-mju.2025.34

Issue

Section

Research Article