Efficiency Test in Coccidiosis Prevention Caused by Eimeria tenella of Penergetic-t Broiler® and Cassia Essential Oil Products in Broilers
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Abstract
This experiment was conducted to investigate the efficiency of Penergetic-t Broiler® product (PB) and cassia essential oil product (COP) in single and combination uses for Coccidiosis prevention. A total of 96 one-day old broilers was kept in floor-pen and offered a commercial diet until 13 days old. Afterwards, broilers were allocated into 8 groups and 6 replicates with 2 birds per each and randomly assigned to eight treatments as following: group 1 was non-challenged with E. tenella and without anticoccidial products supplementation, group 2-8 were challenged groups with 2,500 oocysts and without anticoccidial product (group 2), dietary supplementation with 33 ppm robenidine (group 3), water administration with 0.20 µl/ml COP (group 4), 500 ppm PB through drinking water (group 5), and in combination with 0.20 µl/ml COP (group 6), dietary supplemention with 2,000 ppm PB(group 7) and in combination with 0.20 µl/ml COP through drinking water (group 8). During an experiment, cecal tissues were collected from one bird per each replicate at 7 and 14 post-challenge for histopathological lesion scoring and feces were collected for oocysts shadding enumeration. Results showed that at 7 days after challenge, combination uses of 2,000 ppm PB in diet and 0.20 µl/ml COP through drinking water showed the best efficiency with the low histopathological lesion scoring, whereas at 14 days post challenge, water application with 500 ppm PB and 0.20 µl/ml COP provided the best efficiency to recover caecal tissues after E. tenella infection. In conclusion, the combination uses of Penergetic-t Broiler® and cassia essential oil product can be an alternative for Coccidiosis prevent in broilers.
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