Introgression of Xa21 gene for bacterial blight resistance into the rice cultivar RD 6 (Oryza sativa. L) using marker assisted selection
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Abstract
The rice cultivar RD 6 is the most famous variety planted and consumed in the North and North-East of Thailand. However this cultivar is susceptible to bacterial blight (BB) caused by Xanthomonas oryzae pv. oryzae(Xoo) The objective of this study was to increase resistance to bacterial blight resistance in RD6 for through backcrossing method and marker assisted selection. Resistance gene Xa21 from the resistant cultivar IRBB21 was introgressed into RD6 using sequence tagged sites (STS) marker STSBB21-11 that is tightly linked to Xa21 (0.1 cM) for foreground selection. Nine selected BC2F2:3 lines together with their parental lines and standard check varieties were evaluate for the resistance using artificial inoculation under greenhouse condition against three of Xoo isolates (CN2-1, MS1-2 and NY1-1). Two BC2F2:3 introgression lines showed higher resistance against the three bacteria isolates than RD6. The line BC2F2:3 2-17-71-6 was 2.60 – 3.60 cm. in average lesion length and BC2F2:3 2-19-86-9 was 2.43 – 3.50 cm. which were greater than IRBB21 (4.20 – 5.43 cm.) and RD6 (9.57 – 12.90). The two selected lines will be evaluated for agronomic traits and yield subsequently. This study was successful in breeding the rice cultivar RD6 for bacterial blight resistance. The introgression lines are promising lines and could be further utilized as material for gene pyramiding to achieve horizontal resistance.
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