Shoots Induction from In Vitro Node of Teak

Abstract

This research studied the effects of MS solid medium supplemented with BA, TDZ, and two
additive substances, silver nitrate and adenine sulfate, on shoot proliferation of in vitro teak node explants.
The teak node explants were cultured on MS medium supplemented with 0 - 12.0 mg/l BA. The CRD with
5 replications, each of 2 nodes were applied. The explants were incubated for 4 weeks at 55 µM/m2
/s 16
h light, 25±2 ºC, with two weeks’ interval of subculture. The results demonstrated that higher shoot
proliferation observed when BA concentration increased in the range of 1.0-6.0 mg/L but decreased at
higher concentration of 12 mg/L BA. The treatment using 0.0-6.0 mg/L BA in concerted with 0.1-0.3 mg/L
TDZ revealed inadequate result in which less shoot proliferation was obtained compared with only BA

supplemented. The high variation in shoot proliferation was also observed along with small, glassy
appearance with yellowish green shoots. For the treatment that silver nitrate was added to the MS medium
supplemented with 3.0 mg/L BA, the shoot proliferation was similar to that of no silver nitrate added but
short and bunchy shoots were observed. The treatment of MS medium containing 3.0 mg/L BA
supplemented with adenine sulfate was revealed not necessary because at the low concentration (10-20
mg/L), the shoot proliferation was similar to that of no adenine sulfate added. Furthermore, the adenine
sulfate added at the higher concentrations caused the high variation in shoot proliferation with the grassy
appearance observed.