Effect of Seed Coating with Rhodamine B on Seed Quality and Fluorescence for Anti-Counterfeiting of Tomato Seed
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Abstract
Seeds coated with fluorescent compound is an alternative method for identity and providing
securing for the high value seeds. The objective of this experiment was examined seeds coating of
rhodamine B fluoresceting on seed and seedling of tomato. The experiment was conducted at Seed
Technology Section of Seed Processing Plant, Faculty of Agriculture, and tested detection of the
fluorescence on the seed at Central Laboratory, Faculty of Technology Khon Kaen University. Seed
coating substance were prepared by using 5% of Polyvinylpyrrolidone (K30) as a polymer. After that,
rhodamine B was added at four different label rates: 0.125, 0.150, 0.175 and 0.200% per 100 ml of polymer
solution subsequently, tomato seeds were coated used of the Centric Coater, Model SKK 10. After coating
process, the coated seeds were divided into 2 parts. The first part was evaluated and detected for
fluorescence on seeds and the 14-days seedlings of tomato using hand-UV (Model UVGL-58; λ=365 nm.)
and Spectrophotometer. The second part was evaluated seed quality after coating. The results showed
that the coated seeds with rhodamine B had orange fluorescence light both on seeds and the 14-days
seedlings. Additionally,the coated seed with rhodamine B had no effects on seed germination and vigor
when tested under laboratory and greenhouse conditions. The conclusion of this experiment is rhodamine
B can be used as seed coating substance for identity of tomato seeds. In addition, it can be detected the
fluorescent of rhodarmine B on the seedlings.
Article Details
King Mongkut's Agricultural Journal
References
พันธุ์มะเขือเทศลูกผสม. แก่นเกษตร 42 ฉบับพิเศษ 1.
บุญมี ศิริ และพรทิวา สิมมะคร. 2556. ศึกษาการสร้างสารเคลือบร่วมกับสารเรืองแสงต่อคุณภาพเมล็ดพันธุ์มะเขือเทศ. การประชุมวิชาการ
เมล็ดพันธุ์พืชแห่งชาติ ครั้งที่ 10 หน้า 111-117 วันที่ 20-24 พฤษภาคม 2556 ณ โรงแรม Hansa JB Hotel, จังหวัดสงขลา.
พจนา สีขาว, พัฒนา ธีรพรชัยสิทธิ์ และบุญมี ศิริ. 255. การเคลือบเมล็ดด้วยไรโบฟลาวินต่อเอกลักษณ์และคุณภาพของเมล็ดพันธุ์แตงกวา
ลูกผสม. การประชุมวิชาการเมล็ดพันธุ์พืชแห่งชาติ ครั้งที่ 10 หน้า 46-54.
สมาคมการค้าเมล็ดพันธุ์ไทย. 2555. ชูยุทธศาสตร์บูมการค้าอุตสาหรรมเมล็ดพันธุ์ปี 2554 สู่ฮักเมล็ดพันธุ์เอเชีย. สืบค้นข้อมูลเมื่อ
4 มกราคม 2557 จาก http://www.thannews.th.com/index.phpoption=com_content&view=article&id=51753:2554-&
catid=171:pr&Itemid=512.
Almeida, C de., Rocha, C.D.R. and Razera, L.F. 2005. Polymer coating, germination and vigor of broccoli seed. [Online].
Available at: http://www.scielo.br/scielo.php. cited Dec 15,2005.
Cai, Y.L. 2009. Several new anti-counterfeiting technologies for packaging. Shanghai Packaging 2: 40–41.
Gao, H. and Zhou, R. 2005. The development trend of seed anti-counterfeiting packaging. Seed World 9: 6–7.
Guan, Y., Hu, J., Li, Y., Ma, W. and Zheng, Y. 2011. A new anti-counterfeiting method: fluorescent labeling by safranine T
in tobacco seed. Acta Phys. Plant 33: 1271–1276.
Guan, Y., Wang, J., Hu, J., Li, Y., Ma, W., Hu, W. and Zhu, S. 2013. Pathway to keep seedsecurity: the application of fluorescent
to identify true and fake pelleted seed in tobacco. Ind. Crop Prod. 45, 367–372.
Hapner, S.J.and Hapner, K.D. 1978. Rhosamine immune his to fluorescence applied to plant tissue. J Histochem Cytochem
26:478-482.
Remya, N.A.C., Poulose Y.N., Yasuhiko, Y.T.M.D. and Sakthi, K. 2011. Uptake of FITC labeled silica nanoparticles and quantum
dots by rice seedlings: Effects on seed germination and their potential as biolabels for plants J. Fluoresc. 21: 2057–2068.
Sellei, J. 194. Further growth experiments with fluorescent dyes. Growth 5: 27–52.
Shi, Y.X., Hareland, G.A. and Appolonia, B.L.D. 2001. The use of fluorescent dye to study the movement of water into wheat
kernels. J Zhengzhou Institute of Technol 22:17–20
Tian, Y., Wang, Q., Hu, J., Wang J. and Guan Y. 2013. Application of fluorescent dyes for falsification-preventing of pea
seeds (Pisum sativum L). Australian Journal of Crop Science 7(1): 147-151.
Tian Y.B., Zhan, L., Fei, H., Yajing, G., Shuijin, Z. and Jin, H. (2014) A novel anti-counterfeiting method: Application and
decompositionof RB for broad bean seeds (Vicia faba L.) Industrial Crops and Products 61: 278–283
Wang, Z.H. 2009. Anti-counterfeiting technologies grow by leaps and bounds in the market. Print Today 2: 76–79.
Waser, N.M. and Price, M.V.1982. A comparison of pollen and fluorescent dye carryover by natural pollinators of Ipomopsis
aggregata (Polemoniaceae). Ecology 63: 1168–1172
Wu, F.S. 1987. Localization of mitochondria in plant cells by vital staining with rhodamine 123. Planta 171: 346–357.
Wu, P., Song, C.H.and Zheng, X.Y. 2007. An advanced seed anti-counterfeiting technology and its application prospects.
Vegetables 11: 34–35.
Zhang, YM., Sun, WY. and Xu, M. 2007. The application of anticounterfeiting packaging to seed industry. China Seed Ind
