Effects of BA and NAA on Growth and Development of Musa sapientum (ABB group) cv ‘Kluai Hin’ in vitro and Effects of Substrate on Growth in vivo

Abstract

The effects of BA (6-benzyladenine) and NAA (1-Naphthaleneacetic acid) on growth and
development of Musa sapientum (ABB group) cv ‘Kluai Hin’ in vitro and effects of substrate on growth in
vivo were studied. The purposes were studied sterilization method, cultured medium on shoot formation,
root formation and substrate media. The experimental design was Completely Randomized Design (CRD).
From the method of treating shoot tips with 3 solutions of sodium hypochlorite was cultured for 4 weeks
found that sterilization with 5% (v/v) of sodium hypochlorite for 10 minutes had the highest survival rate as
100.00±0.00% (p>0.05). After plants were grow up in vitro for 1 month, plants were induced shoot formation
by culturing in solid MS media with BA at 6 different concentrations: 0, 1, 2, 3, 4, 5 and 6 mg/L for 20 weeks.

The results showed that MS medium supplemented with BA 1 mg/L gave the highest shoot formation,
root formation and number of leaves were 41.67±4.17 (p<0.05), 16.67±4.17 (p>0.05), and 1.33±0.17
(p<0.05) percentage, respectively. For root induction in the 6 months of banana was used NAA at 4 different
concentrations: 0, 0.5, 1 and 2 mg/L for 4 weeks. It was found that MS medium supplemented with NAA
at 1 mg/L gave the highest root formation was 71.43±18.44 percentage (p>0.05). For transplanting
in natural conditions, three different substrate cultures were used: peat moss, sand and sand mixed with
coconut flake at 1: 1 ratio for 4 weeks. The peat moss content gave the highest survival rate was
83.33 ± 16.67 percentage (p>0.05).