Genetic Purity Test of Thai Hom Mali Rice Using SSR Molecular Markers
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Abstract
Thai Hom Mali Rice (THMR) is recognized for its premium quality in the global market. One of the significant problems of a decrease in the quality of THMR is an admixture of other rice varieties. To maintain the popularity of such rice by targeting the upper market, The Nation Bureau of Agricultural Commodity and Food Standards issued Thai Hom Mali Rice standards stating that must be Thai Hom Mali Rice not less than 92%. Several methods have been employed to enforce this standard and showed less possibility of detecting adulterants. However, DNA-based methods have been proven to purge the adulteration problem. This research reports that 5 SSR primers, RM213, RM336, RM337, RM474, and BV210145, were selected for genetic purity test in THMR. In addition, a simplified rice grain DNA extraction protocol by 0.1M NaOH solution in a single microcentrifuge tube. It was found that there was good DNA that was enough PCR analysis. Furthermore, the appropriate sample size for mixing seeds detection was evaluated at a confidence interval level of 95 percent.
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King Mongkut's Agricultural Journal
References
Apanich, N. (2004). Seed testing. Department of Agriculture, Bangkok (Thailand). 6-17.
Apanich, N., & Urairong, H. (2004). Improving verification method for Thai Hom Mali Rice and Pathum Thani rice variety. Department of Agriculture, Bangkok (Thailand). 1-13.
Chamarerk, V., Cobelli, P., Jairin, J., Mekwattanakarn, P., Leelakud, P., & Wongnongwa, J. (2016). Development of molecular markers for purity testing in Thai Jasmine rice. Advances in Ecological and Environmental Research. 35-44.
Chamarerk, V., Mekwatanakarn, P., Cobelli, P., Leelakud, P.., & Kawichai, R. (2011). Purity test of Thai Hom Mali Rice samples from retail and wholesale markets in People Republic of China using DNA markers. Thai Rice Research Journal. 5(1), 50-58.
Cobelli, P., Chamarerk, V., & Mekwatanakarn, P. (2007). Rice varietal purity test by using molecular marker. Thai Rice Research Journal. 1(1), 44-51.
Cobelli, P., Chamarerk, V., Mekwatanakarn, P., Leelakud, P., & Wangsomboondee T. (2016). SSR Markers for varietal genetic purity testing of rice. Thai Rice Research Journal. 7(2), 77-91.
Doyle, J. J., & Doyle, J. (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin. 19(1), 11-15.
International Seed Testing Association. (1996). International Rules for Seed Testing. Zurich: International Seed Testing Association.
Kongseree, N. (1999). Adulteration of high amylose rice in Hom Mali rice. In Rice and temperate cereal crops annual conference 1999. (pp.47). Suphan Buri: Rice Research Institute.
Ma, Z., Wang, Y., Wei, W., & Ru, Z. (2019). A new harmless, high-throughput endosperm-based DNA extraction method for wheat. Ciência Rural, Santa Maria. 49(9), 1-10.
Miles, S. R. (1963). Handbook of tolerances and of measures of precision for seed testing. In Proceedings of the International Seed Testing Association. (pp. 130–156). Bassersdorf: International Seed Testing Association (ISTA).
Ngorian, S., Urairong, H., A-phanit, N., & Pitaktansakul, R. (2007). DNA fingerprint of new Thai rice varieties. Department of Agriculture, Bangkok (Thailand). 524.
Saengkiew, J., & Srikaew, K. (2012). Test kit for determination of contaminated Hom Mali rice. In Extended abstracts of the 2nd national rice research conference. (pp. 694). Bangkok: National Science and Technology Development Agency.
Tamari, F., Hinkley, C. S., & Ramprashad, N. (2013). A companion of DNA extractor methods using Petunia hybrids Tissues. Journal of Biomolecular Techniques. 24(3), 113–118.
Vejchasarn, P., Jairin, J., Phansenee, Y., Suthanthangjai, A. Danaisilichaichon, C., Narawatthana, S., Cobelli, P., & Chamarerk, V. (2019). Application of next-generation sequencing technologies for purity testing in Thai jasmine rice. Thai Rice Research Journal. 10(2), 59-65.
Verma, D., Srivastav, P., & Nadaf, A. (2018). Aromatic rice from different countries; An overview. In Verma, D. K. & Srivastav, P. P. (Eds.), Science and Technology of Aroma, Flavor, and Fragrance in Rice. (pp. 35-58). New York: Apple Academic Press.
Wen-yyue, C., Hai-rui, C., Jin-song, B. A. O., Xiang-sheng, Z. H. O. U., & Qing-yao, S. H. U. (2006). A simplified rice DNA extraction protocol for PCR analysis. Rice Science. 13(1), 67.
Yoshihashi, T. (2000). Simple and rapid DNA extraction from milled rice and its application to Thai aromatic rice (Oryza sativa L.) variety, Khao Dawk Mali 105. JIRCAS Journal. 8, 41-47.
