Effect of NAA and TDZ on In Vitro Plantlet Induction of Cryptocoryne Wendtii “Brown”
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Abstract
The exotic aquatic plant, Cryptocoryne wendtii “Brown”, can develop into an economic crop with a continually increasing export value. Thailand is unable to produce to meet the demand. Therefore, this experiment aimed to study the optimum concentrations of growth regulators on plantlet induction. The sterile apical bud was cultured on semi-solid MS media (Murashige and Skoog, 1962) supplemented with 1-naphthaleneacetic acid (NAA) at 0, 0.1, 0.2, 0.3 mg/L and thidiazuron (TDZ) at 0, 1, 2, 3 mg/L. In the sixth week, the semi-solid MS medium which contains 0.1 mg/L NAA and 2 mg/L TDZ showed the maximum new plantlets (67.92±19.61 plantlets/explant), leaves (67.42±19.46 leaves/explant) and height (28.65±1.55 mm./plantlet), had the highest average significance (P<0.05). Micropropagation of C. wendtii “Brown” in optimum culture medium could increase the volume within a shorter period and enhances the potential for quality and value-added to aquatic plant production.
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King Mongkut's Agricultural Journal
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