Effects of Different Concentrations of BA with NAA on Shoot Proliferation and Acclimatization of Niaw Dam Mo 37 Rice
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Abstract
Niaw Dam Mo 37 rice has many important benefits, such as reducing cholesterol, inhibiting gastric acid secretion, helping blood circulation, slowing down the degeneration of body cells, and inhibiting the growth of lung cancer cells. In the present, no authors have reported on tissue culture for the propagation and improvement of Niaw Dam Mo 37. Thus, this study aimed to investigate the germination of mature seeds, multiple shoot formation from culturing shoot tip in liquid oil palm culture medium (OPCM) with plant growth regulators and acclimatization of complete plantlets to field conditions. The results revealed that seed germination in 1 week could be divided into 3 types: shoot alone, root alone, and both shoot and root. Total germination after 7 days of cultures was 85.5 percent. For multiple shoot formation, the results showed that shoot tip culture in liquid OPCM medium supplemented with 0.5 mg/L NAA and 1.5 mg/L BA gave the highest number of shoots at 7.6 shoots, significantly different (p<0.05) from other treatments. For acclimatization, plantlets cultured on medium with 0.5 mg/L NAA had the highest survival rate at 100 percent. The investigation’s findings can be used to provide fundamental knowledge for propagation, breeding and plant genetic conservation of Niaw Dam Mo 37 rice in the future.
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King Mongkut's Agricultural Journal
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