The Application of Chlorophyll Absorbents to Enhance Efficiency of Rice ragged stunt virus Detection in Plant Sap by Dot-Immunobinding Assay

Abstract

The detection of the minimum time and some parts of rice cultivar Taichung Native 1 (TN1) which
were transmitted Rice ragged stunt virus (RRSV) by brown planthopper (BPH, Nilaparvata lugens).
Dot-immunobinding assay (DIBA) technique could use to detect RRSV in asymptomatic TN1 rice. After
rice plants were infested within 15 days and could be detected in the sap of both leaves and stems at 1:5
dilution. The RRSV could be detected in the sap of both leaves and stems at 1:500 dilution and root at 1:10
dilution after the plant was infested with the vector for 60 days. In addition, the DIBA technique was
prepared the plant sap by using chlorophyll absorbents. Magnesium oxide and aluminium oxide were used
to detect RRSV in TN1 rice sap with the highest dilutions of infected rice sap at 1:1,500 in both leaves and
stems, whereas the bentonite and activated carbon were detected RRSV with the highest dilutions of

infected rice sap at 1:1,000. For the rice sap from the root, the comparison between the magnesium oxide
and bentonite and the aluminium oxide and activated carbon can be detected RRSV with the highest
dilutions of infected rice sap at 1:50 and 1:100 respectively. The application of chlorophyll absorbents for
detection RRSV in infected plant sap by DIBA can be detected RRSV concentration which were three-fold
increase from the original plant sap of both leaves and stems and ten-fold increase from the original plant
sap of the root. Therefore, the use of chlorophyll absorbents can enhance the sensitivity of the DIBA
technique to be more effective.