The Enhancement of Plumbagin Production Efficiency from Hairy Root of Plumbago indica L. by Salicylic Acid and Benzoic Acid
Article Sidebar
Published:
Sep 19, 2019
Keywords:
Plumbago indica L. hairy root plumbagin salicylic acid benzoic acid
Main Article Content
Abstract
Plumbago indica L. is a medicinal plant which the roots are the main source of plumbagin. Such
plumbagin possesses the anticancer, antibacterial and antifungal activities. This cultivation of P. indica
hairy root, therefore, aimed to enhance plumbagin production of P. indica hairy root using elicitors.
Salicylic acid and benzoic acid with final concentration of 10-40 mM were added into the ½ MS liquid
medium which 15-day-old hairy roots were cultured in. The results reveal that salicylic acid with the final
concentration of 20 mM was the most promising elicitor for stimulating the plumbagin production from hairy
root and the release of plumbagin into the medium on the first day-after-elicitation (93.64 mg/l; 4.4 times
more than control). While using of benzoic acid, it slightly stimulated the plumbagin production and no
plumbagin released into the medium. The highest plumbagin accumulated in hairy roots was 41.78 mg/l
(1.4 times over the control) on the 7th day-after-elicitation.
Article Details
How to Cite
จันทร์สุเทพ ศ., จันทร์เปรม ส., & จันทร์เปรม เ. (2019). The Enhancement of Plumbagin Production Efficiency from Hairy Root of Plumbago indica L. by Salicylic Acid and Benzoic Acid. King Mongkut’s Agricultural Journal, 37(3), 436–445. retrieved from https://li01.tci-thaijo.org/index.php/agritechjournal/article/view/217054
Section
Research Articles
King Mongkut's Agricultural Journal
References
Agilent Technologies. 2001. Agilent Chemstation (Computer Program). Agilent Technologies, USA.
Buitelaar, R. and J. Tramper. 1992. Strategies to improve the production of secondary metabolites with plant cell culture.
J. Biotech. 23: 111-141.
Chong, J., M.A. Pierrel, R. Atanassova, D.W. Reichhart, B. Fritig and P. Saindrenan. 2001. Free and conjugated benzoic
acid in tobacco plants and cell cultures: induced accumulation upon elicitation of defense responses and role as
salicylic acid precursors. Plant Physiol. 125: 318-328.
Chung, I.M., M. Thiruvengadam, K. Rekha and G. Rajakumar. 2016. Elicitation enhanced the production of phenolic
compounds and biological activities in hairy root cultures of bitter melon (Momordica charantia L.). Braz. Arch.
Biol. Technol. 59: e160393.
Chuntaratin, P. 2006. Production of Plumbagin by Hairy Root, Callus and Cell Suspension Cultures of Plumbago indica
L. Ph.D. Thesis. Kasetsart University, Thailand. 133p.
Ding, J., S. Shi, B.H. Jiang, Y.H. Yang, J. Huang, H.G. Shen, K. Xia and J. Jiang. 2004. Effect of methyl jasmonate with
indol-3-acetic acid and 6-benzylaminopurine on the secondary metabolism of cultured Onosma paniculatum cells.
In Vitro Cell. Dev.-Pl. 40: 581-585.
Georgiev, M.I., A.I. Pavlov and T. Bley. 2007. Hairy root type plant in vitro systems as sources of bioactive substances. Appl.
Microbiol. Biotechnol. 74: 1175-1185.
Gharechahi, J. , M. Khalili, T. Hasanloo and G.H. Salekdeh. 2013. An integrated proteomic approach to decipher the effect
of methyl jasmonate elicitation on the proteome of Silybum marianum L. hairy roots. Plant Physiol.Biochem.
70: 115–122.
Hao, X., M. Shi, L. Cui, C. Xu, Y. Zhang and G. Kai. 2015. Effects of methyl jasmonate and salicylic acid on tanshinone
production and biosynthetic gene expression in transgenic Salvia miltiorrhiza hairy roots. Biotechnol. Appl. Bioch.
62:24–31.
Hassan, S.T.S., K. Berchová-Bímová and J. Petráš. 2016. Plumbagin, a plant-derived compound, exhibits antifungal
combinatory effect with amphotericin b against Candida albicans clinical isolates and anti-hepatitis C virus activity.
Phytother. Res. 30: 1487-1492.
Hwang, S.J. 2006. Baicalin production in transformed hairy root clones of Scutellaria baicalensis. Biotechnol. Bioproc.
Eng. 11: 105-109.
Jeong, G.T., D.H. Park, H.W. Ryu, B. Hwang, J.C. Woo, D. Kim and S.W. Kim. 2005. Production of antioxidant compounds
by culture of Panax ginseng C.A. Meyer hairy root. Appl. Biochem. Biotech. 121: 1147-1157.
KrstićMilošević, D., T. Janković, B. Uzelac, D. Vinterhalter and B. Vinterhalter. 2017. Effect of elicitors on xanthone
accumulation and biomass production in hairy root cultures of Gentiana dinarica. Plant Cell Tiss. Organ Cult. 130:
631-640.
Liu, Y., Y. Cai, C. He, M. Chen and H. Li. 2017. Anticancer properties and pharmaceutical applications of plumbagin:
a review. Am. J. Chin. Med. 45: 423-441.
Malarz, J., A. Stojakowska and W. Kisiel. 2007. Effect of methyl jassmonate and salicylic acid on sesquiterpene lactone
accumulation in hairy roots of Chichorium intybus. Acta Physiol. Plant. 29: 127-132.
Mehmetoglu, U. and W.R. Curtis. 1997. Effect of abiotic inducers on sesquiterpene synthesis in hairy root and
cell-suspension cultures of Hyoscyamus muticus. Appl. Biochem. Biotech. 67: 71-77.
Meuwly, P., W. Molders, A. Buchala and J.P. Metraux. 1995. Local and systemic biosysthesis of salicylic acid in infected
cucumber plants. Plant Physiol. 109: 1107-1114.
Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures.
Physiol. Plant. 15: 473-497.
Nair, S.V., G. Baranwal, M. Chatterjee, A. Sachu, A.K. Vasudevan, C. Bose, A. Banerji and R. Biswas. 2016. Antimicrobial
activity of plumbagin, a naturally occurring naphthoquinone from Plumbago rosea, against Staphylococcus aureus and
Candida albicans. Int. J. Med. Microbiol. 306: 237-248.
Pitta-Alvarez, S.I., T.C. Spollansky and A.M. Giulietti. 2000. The influence of different biotic and abiotic elicitors on the
production and profile of tropane alkaloids in hairy root cultures of Brugmansia candida. Enzyme Microb. Technol.
26: 252-258.
Pradeepa, V., Sathish-Narayanan, S., Kirubakaran, S.A., Senthil-Nathan, S., 2014. Antimalarial efficacy of dynamic
compound of plumbagin chemical constituent from Plumbago zeylanica Lin (Plumbaginaceae) against the malarial
vector Anopheles stephensi Liston (Diptera: Culicidae). Parasitol. Res. 113, 3105–3109.
Schenk, P.M., K. Kazan, I. Wilson, J.P. Anderson, T. Richmond, S.C. Somerville and J.M. Manners. 2000. Coordinated plant
defence responses in Arabidopsis revealed by microarray analysis. Proc.Natl. Acad. Sci. USA. 97: 11655-11660.
Tatreerod, S., P. Saralamp and S. Chanprame. 2003. Hairy root induction and culture of Plumbago indica L. In The 3rd World
Congress on Medicinal and Aromatic Plants for Human Welfare. Chiang Mai, Thailand. pp. 306.
Vasconsuelo, A. and R. Boland. 2007. Molecular aspects of the early stages of elicitation of secondary metabolites in plants.
Plant Sci. 172:861–875.
Buitelaar, R. and J. Tramper. 1992. Strategies to improve the production of secondary metabolites with plant cell culture.
J. Biotech. 23: 111-141.
Chong, J., M.A. Pierrel, R. Atanassova, D.W. Reichhart, B. Fritig and P. Saindrenan. 2001. Free and conjugated benzoic
acid in tobacco plants and cell cultures: induced accumulation upon elicitation of defense responses and role as
salicylic acid precursors. Plant Physiol. 125: 318-328.
Chung, I.M., M. Thiruvengadam, K. Rekha and G. Rajakumar. 2016. Elicitation enhanced the production of phenolic
compounds and biological activities in hairy root cultures of bitter melon (Momordica charantia L.). Braz. Arch.
Biol. Technol. 59: e160393.
Chuntaratin, P. 2006. Production of Plumbagin by Hairy Root, Callus and Cell Suspension Cultures of Plumbago indica
L. Ph.D. Thesis. Kasetsart University, Thailand. 133p.
Ding, J., S. Shi, B.H. Jiang, Y.H. Yang, J. Huang, H.G. Shen, K. Xia and J. Jiang. 2004. Effect of methyl jasmonate with
indol-3-acetic acid and 6-benzylaminopurine on the secondary metabolism of cultured Onosma paniculatum cells.
In Vitro Cell. Dev.-Pl. 40: 581-585.
Georgiev, M.I., A.I. Pavlov and T. Bley. 2007. Hairy root type plant in vitro systems as sources of bioactive substances. Appl.
Microbiol. Biotechnol. 74: 1175-1185.
Gharechahi, J. , M. Khalili, T. Hasanloo and G.H. Salekdeh. 2013. An integrated proteomic approach to decipher the effect
of methyl jasmonate elicitation on the proteome of Silybum marianum L. hairy roots. Plant Physiol.Biochem.
70: 115–122.
Hao, X., M. Shi, L. Cui, C. Xu, Y. Zhang and G. Kai. 2015. Effects of methyl jasmonate and salicylic acid on tanshinone
production and biosynthetic gene expression in transgenic Salvia miltiorrhiza hairy roots. Biotechnol. Appl. Bioch.
62:24–31.
Hassan, S.T.S., K. Berchová-Bímová and J. Petráš. 2016. Plumbagin, a plant-derived compound, exhibits antifungal
combinatory effect with amphotericin b against Candida albicans clinical isolates and anti-hepatitis C virus activity.
Phytother. Res. 30: 1487-1492.
Hwang, S.J. 2006. Baicalin production in transformed hairy root clones of Scutellaria baicalensis. Biotechnol. Bioproc.
Eng. 11: 105-109.
Jeong, G.T., D.H. Park, H.W. Ryu, B. Hwang, J.C. Woo, D. Kim and S.W. Kim. 2005. Production of antioxidant compounds
by culture of Panax ginseng C.A. Meyer hairy root. Appl. Biochem. Biotech. 121: 1147-1157.
KrstićMilošević, D., T. Janković, B. Uzelac, D. Vinterhalter and B. Vinterhalter. 2017. Effect of elicitors on xanthone
accumulation and biomass production in hairy root cultures of Gentiana dinarica. Plant Cell Tiss. Organ Cult. 130:
631-640.
Liu, Y., Y. Cai, C. He, M. Chen and H. Li. 2017. Anticancer properties and pharmaceutical applications of plumbagin:
a review. Am. J. Chin. Med. 45: 423-441.
Malarz, J., A. Stojakowska and W. Kisiel. 2007. Effect of methyl jassmonate and salicylic acid on sesquiterpene lactone
accumulation in hairy roots of Chichorium intybus. Acta Physiol. Plant. 29: 127-132.
Mehmetoglu, U. and W.R. Curtis. 1997. Effect of abiotic inducers on sesquiterpene synthesis in hairy root and
cell-suspension cultures of Hyoscyamus muticus. Appl. Biochem. Biotech. 67: 71-77.
Meuwly, P., W. Molders, A. Buchala and J.P. Metraux. 1995. Local and systemic biosysthesis of salicylic acid in infected
cucumber plants. Plant Physiol. 109: 1107-1114.
Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures.
Physiol. Plant. 15: 473-497.
Nair, S.V., G. Baranwal, M. Chatterjee, A. Sachu, A.K. Vasudevan, C. Bose, A. Banerji and R. Biswas. 2016. Antimicrobial
activity of plumbagin, a naturally occurring naphthoquinone from Plumbago rosea, against Staphylococcus aureus and
Candida albicans. Int. J. Med. Microbiol. 306: 237-248.
Pitta-Alvarez, S.I., T.C. Spollansky and A.M. Giulietti. 2000. The influence of different biotic and abiotic elicitors on the
production and profile of tropane alkaloids in hairy root cultures of Brugmansia candida. Enzyme Microb. Technol.
26: 252-258.
Pradeepa, V., Sathish-Narayanan, S., Kirubakaran, S.A., Senthil-Nathan, S., 2014. Antimalarial efficacy of dynamic
compound of plumbagin chemical constituent from Plumbago zeylanica Lin (Plumbaginaceae) against the malarial
vector Anopheles stephensi Liston (Diptera: Culicidae). Parasitol. Res. 113, 3105–3109.
Schenk, P.M., K. Kazan, I. Wilson, J.P. Anderson, T. Richmond, S.C. Somerville and J.M. Manners. 2000. Coordinated plant
defence responses in Arabidopsis revealed by microarray analysis. Proc.Natl. Acad. Sci. USA. 97: 11655-11660.
Tatreerod, S., P. Saralamp and S. Chanprame. 2003. Hairy root induction and culture of Plumbago indica L. In The 3rd World
Congress on Medicinal and Aromatic Plants for Human Welfare. Chiang Mai, Thailand. pp. 306.
Vasconsuelo, A. and R. Boland. 2007. Molecular aspects of the early stages of elicitation of secondary metabolites in plants.
Plant Sci. 172:861–875.
