The Preliminary Study on Antibacterial Efficacy of 4 Organic Acids on Mastitis Pathogens in Dairy Cattle
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Abstract
The aim of this study was to evaluate the antimicrobial efficacies of formic acid, lactic acid, malic acid and citric acid against mastitis-caused pathogens. Pathogens used in this study were Klebsiella spp, Escherichia coli, Streptococcus dysgalactiae, Streptococcus uberis and Staphylococcus aureus. Minimum bactericidal concentration (MBC) and reduction of total bacterial count were examined. The results revealed that the minimum bactericidal concentration (MBC) of formic acid against all tested pathogens was 0.191%v/v indicating the best bactericidal performance. In comparison, the following orders were 10.625% lactic acid, 12.37% malic acid and 12.43% citric acid, respectively. These acids at their best solutions were then used to test their antimicrobial efficacies on teats of dairy cattle. It was found that 12.43% citric acid, as well as 10.625% lactic acid, showed the best performance due to the reduction rate at 1.15 log10CFU/ml.(P<0.05) and 1.69 log10 CFU/ml (P=0.32), respectively. On the contrary, formic acid and malic acid were significantly unable to reduce the total bacteria compared with the control group. In conclusion, the results in this study revealed that citric acid and lactic acid have potential antimicrobial effects against mastitis-caused pathogens in dairy cattle.
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King Mongkut's Agricultural Journal
References
Boddie, R. L. & Nickerson, S. C. (1988). Efficacy of a Fatty Acid-Lactic Acid Postmilking Teat Germicide in Reducing Incidence of Bovine Mastitis. Journal of Food Protection. 51(10), 799-801.
Boonyayatra, S. & Chaisri, W. (2004). Incidence and prevalence of subclinical mastitis in small holder dairy farm in Chiang mai province, Thailand. Chiang Mai Veterinary medicine Journal. 2, 25-30. (in Thai).
Cha, C. N., Park, E. K., Jung, J. Y., Yoo, C. Y., Kim, S. & Lee, H. J. (2016). Bactericidal Efficacy of a disinfectant spray containing a grapefruit-seed extract, citric acid, malic acid and benzalkonium chloride against Salmonella Typhimurium and Brucella ovis. Journal of Food Hygiene and Safety. 31(4), 299-303.
Clinical and Laboratory Standards Institute. (2017). Performance standards for antimicrobial susceptibility testing; twenty three informatinal supplement. CLSI document M100-S23. Pensylvania: Clinical and Laboratory Standards Institute.
Eardmusic, S. & Yodmingkwan, P. (2016). Microbiology quality of raw milk in bulk tanks from dairy farms at Cha-am district, Phetchaburi province. Khon Kaen agriculture journal. 44 (suppl 1), 487-493. (in Thai).
Jarassaeng, C., Aiumlamai, S., Wachirapakorn, C., Techakumphu, M., Noordhuizen, J. P. T. M., Beynen & A. C. Suadsong. S. (2012). Risk factors of subclinical mastitis in small holder dairy cows in Khon kaen province. The Thai Journal of Veterinary Medicine. 42(2), 143-151. (in Thai).
Kampa, J., Sukolapong, V., Chaiyotwittakun, A., Rerk-u-suke, A. & Polpakdee, A. (2010). Chronic mastitis in small dairy cattle herds in Muang Khon Kaen. The Thai Journal of Veterinary Medicine. 40(3), 265-272. (in Thai).
Mišeikiene, R., Rudejeviene, J. & Gerulis, G. (2015). Effect of pre-milking antiseptic treatment on the bacterial contamination of cow teats’ skin. Bulgarian Journal of Veterinary Medicine. 18(2), 159-166.
Pavicic, Ž., Cergolj, M., Balenovic, T., Ekert-Kabalin, A. & Valpotic, H. (2008). Influence of udder sanitation on hygienic quality of cow milk. Veterinarski Arhiv. 78, 105-112.
Panyapinyopol, B., Marhaba, T. F., Kanokkantapong, V. & Pavasant. P. (2005). Characterization of precursors to trihalomethanes formation in Bangkok source water. Journal of Hazardous Materials. 120, 229-236. (in Thai).
Rodrigues, P. M. S. M., Esteves da Silva, J. C. G. & Antunes, M. C. G. (2007). Factorial analysis of the trihalomethanes formation in water disinfection using chlorine. Analytica Chimica Acta. 595, 266-274.
Ruegg, P. L. & Dohoo, I. R. (1997). A benefit to cost analysis of the effect of premilking teat hygiene on somatic cell count and intramammary infections in a commercial dairy herd. Canadian Veterinary Journal. 38, 632-636.
Seo Y., Lee, G., Song, S., Kim, K. & Cho, M. (2021). Combinatorial treatment using citric acid, malic acid, and phytic acid for synergistical inactivation of foodborne pathogenic bacteria. Korean J Chem Eng. 38, 826-832.
Soccol, C. R., Vandenberghe, L. P. S., Rodrigues, C. & Pandey, A. (2006). New perspectives for citric acid production and application. Food Technoogy Biotechnology. 44(2), 141-149.
Suriyasathaporn, W. & Chupia, V. (2011). Reduction in numbers of bacteria after pre-milking teat dipping in milking dairy cows. Chiang Mai University Journal of Natural Sciences. 10(2), 301-306. (in Thai).
The National Mastitis Council. (2016). Recommended Mastitis Control Program. The National Mastitis Council. Retrieved from: https://www.nmconline.Org/Docs/NMCchecklistNA.Pdf
Vargas, C. (2017). Organic acids: characteristics, properties and synthesis. New York: Nova Science Publishers, Inc.
