Optimization for Stability of Chlorophyll Pigment from Chara corallina Using Response Surface Methodology

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Published: May 28, 2025
DOI: https://doi.org/10.55003/kmaj.2025.05.28.004
Keywords:
Chara coralline chlorophyll pigment response surface methodology

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Nopparat Mahae
Food Industry Program, Faculty of Science and Fisheries Technology, Rajamangala University of Technology Srivijaya, Trang 92150
Darika Awapak
Food Industrial Technology and Management Program, Faculty of Agro-Industry, Prince of Songkla University, Songkhla, 90110
Donrudee Pichairat
Food Industry Program, Faculty of Science and Fisheries Technology, Rajamangala University of Technology Srivijaya, Trang 92150

Abstract

     This experiment aimed to study the optimum conditions for maintaining the stability of chlorophyll in Chara corallina with sodium bicarbonate (NaHCO3) solution using RSM (Response Surface Methodology).  Factors used in the study were NaHCO3 concentration (X1), reaction temperature (X2) and reaction time (X3).  Chlorophyll concentration (ug.g-1) and green value of chlorophyll (-a*) were analyzed.  The results showed that the R-Square (R2) value of the regression equation coefficients for chlorophyll concentration was 92.98%.  This demonstrated that the concentration of NaHCO3, reaction temperature and reaction time could have explained 92.98% of the variation or change in chlorophyll concentration.  For measuring green values, the results were similar to those for measuring chlorophyll concentration. The optimum conditions for maintaining the stability of chlorophyll were that the NaHCO3 concentration was 0.3 %, the reaction temperature was 90 °C, and the reaction time was 10 minutes. The above conditions yielded the highest chlorophyll concentration (7.647 ug.g-1). The results of this study can be used to maintain the stability of chlorophyll in Chara coralline. Maintaining the stability of chlorophyll allows for more efficient utilization of chlorophyll.

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How to Cite
Mahae, N., Awapak, D. ., & Pichairat, D. . (2025). Optimization for Stability of Chlorophyll Pigment from Chara corallina Using Response Surface Methodology. King Mongkut’s Agricultural Journal, 43(2), 168–179. https://doi.org/10.55003/kmaj.2025.05.28.004
Issue
Vol. 43 No. 2 (2025): May-August
Section
Research Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

King Mongkut's Agricultural Journal

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