Phosphate-Solubilizing Potential of Aspergillus niger TISTR 3254 and Penicillium notatum TISTR 3560 and Their Application with Rice Noodle Wastewater for Compost Production
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Abstract
This research aimed to compare the phosphate rock solubilization efficiency of the fungi Aspergillus niger TISTR 3254 and Penicillium notatum TISTR 3560, as well as the use of their mixed culture to enhance major nutrient contents in compost derived from cattle manure, using discarded rice noodle wastewater (RNW) as a supplementary organic nutrient source. The experiment was conducted at the laboratory scale at 30 °C for 8 days. The results showed that the mixed culture of A. niger TISTR 3254 and P. notatum TISTR 3560 achieved the highest phosphate solubilization, reaching 85.43 mg/L, and reduced the pH from 7.03 to 5.81, which was significantly lower than that obtained from single-strain fermentation (P < 0.05). For compost development, the addition of 2% (v/w) discarded RNW and 5% (w/w) phosphate rock, together with 1.5% (w/w) mixed fungal inoculum, resulted in the highest phosphorus content of 0.93%, along with nitrogen and potassium contents of 1.65% and 3.06%, respectively, which complied with the organic fertilizer standards of the Department of Agriculture. Storage of the compost at room temperature for 15 days led to a slight reduction in major nutrient contents; however, the values remained within the standard limits. The findings indicate that the application of fungi in a co-fermentation system with discarded RNW significantly enhances phosphate solubilization efficiency and compost quality at the 95% confidence level. Moreover, this approach provides a sustainable alternative for agricultural waste utilization, reduces production costs, and is environmentally friendly.
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King Mongkut's Agricultural Journal
References
Aliyat, F. Z., Maldani, M., Guilli, M. E., Nassiri, L., & Ibijbijen, J. (2022). Phosphate-solubilizing bacteria isolated from phosphate solid sludge and their ability to solubilize three inorganic phosphate forms: calcium, iron, and aluminum phosphates. Microorganisms, 10(5), 980–992. https://doi.org/10.3390/microorganisms10050980.
Booali, S., Zoufan, P., & Bavani, M. R. Z. (2024). Effect of biofertilizer containing Thiobacillus bacteria along with different levels of chemical sulfur fertilizer on growth response and photochemical efficiency of small radish plants (Raphanus sativus L. var. shushtari) under greenhouse conditions. Scientia Horticulturae, 327(1), 112835. https://doi.org/10.1016/j.scienta.2023.112835.
Dahunsi, S. O., & Ogunwole, O. J. (2021). Biofertilizer production systems: Industrial insights. In A. Rakshit, V. S. Meena, M. Parihar, H. B. Singh, & A. K. Singh (Eds.), Biofertilizers: Advances in Bio-inoculants, pp. 21–30. Woodhead Publishing. https://doi.org/10.1016/B978-0-12-821667-5.00001-4.
Department of Agriculture. (2010). Organic fertilizer standard (B.E. 2553). Ministry of Agriculture and Cooperatives, Thailand. (in Thai).
Ding, B., Cao, H., Bai, Y., Guo, S., Zhang, J., He, Z., Wang, B., Jia, Z., & Liu, H. (2024). Effect of biofertilizer addition on soil physicochemical properties, biological properties, and cotton yield under water irrigation with different salinity levels in Xinjiang, China. Field Crops Research, 308(1), 109300. https://doi.org/10.1016/j.fcr.2024.109300.
Elias, A. M., Gargalo, C. L., Farinas, C. S., & Gernaey, K. V. (2024). Production of phosphate biofertilizers as a booster for the techno-economic and environmental performance of a first-generation sugarcane ethanol and sugar biorefinery. Journal of Cleaner Production, 471(1), 143400. https://doi.org/10.1016/j.jclepro.2024.143400.
Ferrari, P. F., Bufalini, C., Campardelli, R., Brondolo, A., Ercole, E., Palombo, D., & Perego, P. (2024). Stabilization of microbial strains in long-lasting double emulsions as a new strategy for liquid biofertilizer formulation. Food and Bioproducts Processing, 148(1), 611–618. https://doi.org/10.1016/j.fbp.2024.10.020.
Fernández-Delgado, M., del Amo-Mateos, E., Lucas, S., García-Cubero, M. T., & Coca, M. (2022). Liquid fertilizer production from organic waste by conventional and microwave-assisted extraction technologies: Techno-economic and environmental assessment. Science of The Total Environment, 806(4), 150904. https://doi.org/10.1016/j.scitotenv.2021.150904.
IBM Corp. (2019). IBM SPSS Statistics for Windows (Version 26.0). Retrieved from: https://www.ibm.com/products/spss-statistics
Liao, Z., Boubakri, H., Chen, B., Farooq, M., Lai, Z., Kou, H., & Fan, J. (2025). Biofertilizers as an eco-friendly approach to combat drought stress in plants. Biocatalysis and Agricultural Biotechnology, 64(1), 103510. https://doi.org/10.1016/j.bcab.2025.103510.
Mesa, A. P., Grattz, P. A. C., Vargas, J. J. V., Ríos, L. A., Echeverri, D. O., & Parra, A. M. M. (2025). Feasibility of nitrogen and phosphorus removal from treated wastewater using microalgae and potential microalgae use as biofertilizer. Journal of Water Process Engineering, 70(1), 107023. https://doi.org/10.1016/j.jwpe.2025.107023.
Nawaz, T., Saud, S., Gu, L., Khan, I., Fahad, S., & Zhou, R. (2024). Cyanobacteria: Harnessing the power of microorganisms for plant growth promotion, stress alleviation, and phytoremediation in the era of sustainable agriculture. Plant Stress, 11(1), 100399. https://doi.org/10.1016/j.stress.2024.100399.
Ogbo, F. C. (2010). Conversion of cassava wastes for biofertilizer production using phosphate-solubilizing fungi. Bioresource Technology, 101(11), 4120–4124. https://doi.org/10.1016/j.biortech.2009.12.057.
Sundberg, C., Smårs, S., & Jönsson, H. (2004). Low pH as an inhibiting factor in the transition from mesophilic to thermophilic phase in composting. Bioresource Technology, 95(2), 145–150. https://doi.org/10.1016/j.biortech.2004.01.016.
Thongpradistha, S., Muadsri, T., & Sukkaew, A. (2020). The effect of organic fertilizer formula from Sajor-caju mushroom (Pleurotus sajor-caju) waste on macronutrients. Recent Science and Technology, 12(1), 61–71. (in Thai).
Tian, Y., Liu, Y., Yue, L., Zhao, X., Zhou, Q., Uwaremwe, C., Wang, Y., Chen, G., Sha, Y., Zhang, Y., & Wang, R. (2024). Multi-omics analysis reveals the effects of three application modes of plant growth promoting microbes biofertilizer on potato (Solanum tuberosum L.) growth under alkaline loess conditions. Microbiological Research, 287(1), 127855. https://doi.org/10.1016/j.micres.2024.127855.
