Effects of BAP and NAA on In Vitro Acceleration of Flowering and Growth of Dendrobium lindleyi Steud.
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Abstract
The Dendrobium lindleyi Steud. has yellow-orange flowers and a sweet honey-like scent, making it popular among orchid growers. Propagation of orchids by seeds in nature have a low survival rate, slow growth and flowering. Such limitations are obstacles to the propagation and breeding of orchids. This research aimed to study the effects of 6-benzylaminopurine (BAP) in combination with 1-naphthaleneacetic acid (NAA) on the in vitro growth and flowering of Dendrobium lindleyi. The ten-week-old seedlings were cultured on modified MS medium supplemented with BAP and NAA at 0+0, 0.5+0.5, 0.5+1.0, 0.5+1.5, and 0.5+2.0 mg/L for twenty weeks. The results showed that the modified MS medium supplemented with BAP and NAA at 0.5+1.0 mg/L and 0.5+2.0 mg/L resulted in the highest average number of flowers, which was 0.6 flowers/explant, at the fourth weeks of cultured. The modified MS medium supplemented with BAP and NAA at a concentration of 0.5+0.5 mg/L resulted in the highest average number of shoots (11.9 shoots/explant) and shoot height (2.4 cm). The modified MS medium supplemented with BAP and NAA at a concentration of 0.5+1.5 mg/L resulted in the highest average root number of 18.8 roots/explant. The means were statistically significantly different at the 95% confidence level.
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King Mongkut's Agricultural Journal
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