Effects of Zeatin and IAA on Pepper Embryogenesis by Shed-microspore Culture
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Abstract
Effects of zeatin and IAA on embryogenesis by shed-microspore culture was carried out on pepper genotype 17212 using anthers with 10% purple tip containing a mixed population of mid to uninucleate microspores. Plant growth regulators supplemented in the double layer media were 0.0, 0.1, 0.5, 1.0 mg/L zeatin with 0.0, 0.5, 0.8, 1.8 mg/L IAA. It was found that 0.1 mg/L zeatin and 0.5 mg/L IAA induced significantly highest 40.8% embryo and 35.9% regenerated plants. Ploidy levels of regenerated plants were determined by flow cytometry. It was found that 62% were haploid and 38% were spontaneous doubled haploid. Transferred shed microspore culture can be used efficiently for double haploid production in pepper.
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References
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