Evaluation of Antiphytopathogenic Activity of Endophytic Fungi Isolated from Parasitic Plants and their Hosts
Article Sidebar
Main Article Content
Abstract
The present study was aimed to screen for antimicrobial activity of endophytic fungi isolated from parasitic plants and their hosts (Bouea macrophylla, Manilkara zapota and Morus alba) and to identify the selected potent strains. Forty seven endophytic fungi were grown in five fermentation media, potato dextrose broth PDB, glucose soybean meal broth GSB, malt extract broth MEB, yeast extract sucrose broth YES and Sabouraud broth SBB and incubated on a reciprocal shaker at room temperature (27-30 ºC) for 14 days. The ethyl acetate crude extracts from fermentation broths were tested for antibacterial and antifungal activity against 7 phytopathogenic strains (Ralstonia solanacearum TNCC003291, Xanthomonas citri BS356, Botrytis sp. BS298, Colletotrichum sp. BS351, Fusarium sp. BS288, Phytophthora botryosa BS349 and Pyricularia oryzae BS265) by agar well diffusion method. Dimethylsulfoxide (10%), streptomycin (1 mg/ml) and benomyl (20 mg/ml) were used as control. Crude extracts obtained from endophytic fungi cultured in PDB displayed activity against phytopathogenic bacteria and fungi. While crude extracts obtained from endophytic fungi cultured in GSB, MEB, YES and SBB displayed activity against only phytopathogenic bacteria. The Crude extract of Lasiodiplodia pseudotheobromae MoS5 cultured in GSB and YES showed the most inhibition of R. solanacearum TNCC003291 (12 mm diameter) and X. citri BS356 (22 mm diameter) with minimum inhibitotory concentration (MIC) 2.5 and 5 mg/ml, respectively. While crude extracts of the two endophytes, Colletotrichum sp. MoPIV2 and Phomopsis sp. MoS3/1 cultured in PDB greatest inhibited growth of Fusarium sp. BS288 with 18 and 15 mm inhibition zone diameter and the MIC was 1.25 mg/ml. The MIC of the two extracts that prevented growth of Fusarium sp. BS288 was 8 times lower than the MIC of fungicide benomyl (10 mg/ml). These results indicated that endophytic fungi from parasitic plants and their hosts is a potential source of bioactive compounds that may useful in agriculture.
Article Details
References
คิดคม สเลลานนท์. 2541. ฤทธิ์ลดความดันเลือดของสารสกัดกาฝากมะม่วง (Dendrophthoe pentandra (L.) Miq.) ในหนูขาวความดันเลือดสูง. วิทยานิพนธ์วิทยาศาสตรมหาบัณฑิต. มหาวิทยาลัยขอนแก่น. ขอนแก่น. 130 หน้า.
บุษณีย์ เอี่ยมสีดา และประภาสินี วงษ์ชนะสิทธิ์. 2550. สัณฐานวิทยาและความสามารถของกาฝากต้นหว้าในการยับยั้งแบคทีเรียบางชนิด. ปัญหาพิเศษวิทยาศาสตรบัณฑิต. มหาวิทยาลัยราชภัฏนครราชสีมา. นครราชสีมา. 64 หน้า
วิพรพรรณ์ โพธิตา และพิมพร สุยาว. 2549. การศึกษาเชื้อราสาเหตุโรคขิงและการป้องกันกำจัดโดยใช้สารเคมี. การประชุมทางวิชาการของมหาวิทยาลัยเกษตรศาสตร์ ครั้งที่ 44. (30 มกราคม - 2 กุมภาพันธ์ 2549) มหาวิทยาลัยเกษตรศาสตร์. กรุงเทพฯ. หน้า 497-501.
อภาพร โพธิยอด และเกวลิน คุณาศักดากุล. 2557. การชักนำให้เกิดความต้านทานโรครากเน่าไฟ ทอปธอราในสตรอว์เบอร์รีที่เพาะเลี้ยงเนื้อเยื่อด้วยเชื้อแอกติโนไมซีสต์เอนโดไฟต์. วารสารเกษตร 30(3): 213-222.
de Abreu, L. M., A. R. Almeida, M. Salgado and L. H. Pfenning. 2010. Fungal endophytes associated with the mistletoe Phoradendron perrottettii and its host tree Tapirira guianensis. Mycological Progress 9(4): 559-566.
Alves, A., P. W. Crous, A. Correia and A. J. L. Phillips. 2008. Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae. Fungal Diversity 28(1): 1-13.
Bacon, C. W. and J. F. White. 2000. Microbial Endophytes. Marcel Dekker, New York. 88 p.
Begoude, B. D., B. Slippers, M. J. Wingfield and J. Roux. 2010. Botryosphaeriaceae associated with Terminalia catappa in Cameroon, South Africa and Madagascar. Mycological Progress 9(1): 101-123.
Begoude, B. D., B. Slippers, M. J. Wingfield and J. Roux. 2011. The pathogenic potential of endophytic Botryosphaeriaceous fungi on Terminalia species in Cameroon. Forest Pathology 41: 281-292.
Bussaban, B., S. Lumyong, P. Lumyong, E. H. C. McKenzie and K. D. Hyde. 2001. Endophytic fungi from Amomum siamense. Canadian Journal of Microbiology 47(10): 943-948.
Bussaban, B., S. Lumyong, P. Lumyong, T. Seelanan, D. C. Park, E. H. C. McKenzie and K. D. Hyde. 2005. Molecular and morphological characterization of Pyricularia and allied genera. Mycologia 97(5): 1002-1011.
Calvo, A. M., R. A. Wilson, J. W. Bok and N. P. Keller. 2002. Relationship between secondary metabolism and fungal development. Microbiology and Molecular Biology Reviews 66(3): 447-459.
Chantarasomboon, P., K. Yoshihira, W. K. Natoris, U. Goto and M. Kugo. 1974. Thai medicinal plants. VII. Chemical and pharmacological studies on the constituents of Loranthus pentandrus. Shoyakuguku Zasshi 28: 7-14.
Crawford, K. M., J. M. Land and J. A. Rudger. 2010. Fungal endophytes of native grasses decrease insect herbivore preference and performance. Oecologia 164(2): 431-444.
Devehat, F. L., S. Tomasi, D. Fontanel and J. Boustie. 2002. Flavonols from Scurrula ferruginea Danser (Loranthaceae). Zeitschrift fur Naturforschung 57: 1092-1095.
Druzhinina, I. S., V. Seidi-Seiboth, A. Herrera-Estrella, B. A. Horwitz, C. M. Kenerley, E. Monte, P. K. Mukherjee, S. Zeilinger, I. V. Grigoriev and C. P. Kubicek 2011. Trichoderma: the genomics of opportunistic success. Nature Reviews Microbiol 9(10): 749-759.
Duraipandiyan, V. and S. Ignacimuthu. 2007. Antibacterial and antifungal activity of Cassia fistula L.: An ethnomedicinal plant. Journal of Ethnopharmacology 112(3): 590-594.
García Parisi, P. A., A. A. Grimoldi and M. Omacini. 2014. Endophytic fungi of grasses protect other plants from aphid herbivory. Fungal Ecology 9: 61-64.
Gerke, J. and G. H. Braus. 2014. Manipulation of fungal development as source of novel secondary metabolites for biotechnology. Applied Microbiology and Biotechnology 98(20): 8443-8455.
Griffin, D. H. 1994. Fungal Physiology, 2nd edition. Wiley, New York. 80 p.
Koneman, E. W., S. D. A. Stephens, Jr. V. R. Dowell and H. M. Sommer 1983. Gram positive cocci. Enterococcus-selective mediums. pp. 750-782. In Color Atlasand Textbook of Diagnostic Micobiology, 2nd ed. J.B. Lippincott, Washington, DC.
Li, L., B. Qiao and Y. Yuan. 2007. Nitrogen sources affect Streptolydigin production and related secondary metabolites distribution of Streptomyces lydicus AS4. 2501. Chinese Journal of Engineering 15(3): 403-410.
Phruekphakphom, A. and S. Muunsaan. 1979. Phyochemical Study of Dendrophthoe pentandra Miq. Undergraduate Special Project Report, Mahidol University 54 p.
Powthong, P., B. Jantrapanukorn, A. Thongmee and P. Suntornthiticharoen. 2012. Evaluation of endophytic fungi extract for their antimicrobial activity from Sesbania grandiflora (L.) Pers. International Journal of Pharmaceutical and Biomedical Research 3(2): 132-136.
Prihastuti, H., L. Cai, H. Chen, E. H. C. McKenzie and K. D. Hyde. 2009. Characterization of Colletotrichum species associated with coffee berries in northern Thailand. Fungal Diversity 39: 89-109.
Rogers, J. K., B. C. Morton and J. Mosali. 2011. Plant and endophyte effect on fiber, N and P concentrations in tall fescue. International Journal of Agronomy 1: 1-7.
Slippers, B. and M. J. Wingfield. 2007. Botryosphaeriaceae as endophytes and latent pathogens of woody plants: diversity, ecology and impact. Fungal Biology Reviews 21(2): 90-106.
Sutton, B. C. 1980. The Coelomycetes. Fungi Imperfecti with Pycnidia, Acervuli and Stromata. Commonwealth Mycological Institute, Kew. 696 p.
Suwannarach, N., B. Bussaban, K. D. Hyde and S. Lumyong. 2010. Muscodor cinnamomi, a new endophytic species from Cinnamomum bejolghota. Mycotaxon 114(1): 15-23.
Swofford, D. L. 2002. PAUP*. Phylogenetic Analysis Using Parsimony (*and other methods). Sinauer Associates, Sunderland Massachusetts. 65 p.
Tanakulpakorn, J., A. Pilantanapak and R. Srivibool. 2012. Antifungal activity of endophytic fungi TH121 Isolated from Thespesia populnea against Alternaria brassicicola. Thai Journal of Science and Technology 1: 32-41.
Tao, G., Z. Y. Liu, K. D. Hyde, X. Z. Lui and Z. N. Yu. 2008. Whole rDNA analysis reveals novel and endophytic fungi in Bletilla ochracea (Orchidaceae). Fungal Diversity 33: 101-122.
Ting, A. S. Y., S. Meon, J. Kadir, S. Radu and G. Singh. 2008. Endophytic microorganisms as potential growth promoters of banana. BioControl 53: 541-553.
Weir, B., P. R. Johnston and U. Damm. 2012. The Colletotrichum gloeosporioides species complex. Studies in Mycology 73: 115-180.
Wilson, D. 2000. Ecology of woody plant endophytes. pp. 389-420. In: Bacon, C. W. and White, J. F. (eds.), Microbial Endophytes. Marcel Dekker, New York.
Xie, L., J. Z. Zhang, Y. Wan and D. W. Hu. 2010. Identification of Colletotrichum spp. isolated from strawberry in Zhejiang province and Shanghai city, China. Journal of Zhejiang University Science B (Biomedicine & Biotechnology) 11(1): 61-70.
