Efficiency of Antagonistic Bacterium <I> Bacillus megaterium </I> Strain No.16 for Controlling Sheath Blight Disease of RD6 Rice Variety
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The potential antagonistic bacteria Bacillus megaterium strain No.16 for controlling sheath blight disease of RD6 rice variety caused by Rhizoctonia solani was evaluated. Four isolates of R. solani RSLPN-1, RSLPN-2, RSLPN-3 and RLSPN-4 were obtained from experimental rice paddy fields of Lamphun Land Development Station, Si Bua Ban sub-district, Mueang district, Lamphun province. Dual culture test was determined to investigate the ability of antagonistic bacteria to inhibit mycelial growth of these pathogens for 4 days. This antagonistic B. megaterium showed significantly inhibited of all R. solani isolates at 41.65, 26.78, 26.08 and 42.35%, respectively; especially isolate RLSPN-4 had shown high percentage of growth inhibition. Whereas, the antagonistic B. megaterium showed significantly against mycelial growth of R. solani isolates RSLPN-2 and RLSPN-4 more than 95 % by pour plate test. Mycelial growth inhibition by B. megaterium was observed under compound microscope in either dual culture or pour plate tests. For control of rice sheath blight disease, spraying the granule formulation of B. megaterium on rice plants in the greenhouse was significantly suppressed sheath blight disease of rice caused by R. solani isolate RLSPN-4 when compared to control. These suggested that the antagonistic bacteria B. megaterium have an excellent potential to be used as biocontrol agent of sheath blight disease in rice RD6 variety in laboratory and greenhouse conditions.
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