Induction of Haploid Plants from Ovary Culture of Backcross Line Rathu Heenati/KDML105//Chai Nat 1
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Published:
Jul 31, 2020
Keywords:
Rice ovary culture callus induction plant regeneration haploid plant
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Abstract
Callus induction from the rice ovaries of 2 backcross lines (BC4F3) of a cross Rathu Heenati/KDML 105 //Chai Nat 1 was performed on the N6 medium supplemented with different auxin and cytokinins combinations. Results showed that N6 medium supplemented with 2 mg/L of NAA, 2 mg/L of 2,4-D, 1 mg/L of kinetin, 500 mg/L of CH and 40 g/L of maltose gave the best result for the ovary callus induction, 11.00 and 6.60 % of CH75 and CH71 lines, respectively. Green plantlet regeneration from ovary calli successfully generated at the rate of 7.46 %, when culturing on MS medium supplemented with 2 mg/L of BAP, 0.5 mg/L of NAA, 500 mg/L of CH and 30 g/L of sucrose. Plants derived from ovary showed haploid characteristics. Chromosome number counting and microscopic observation on epidermal peels of expanded leaf confirmed that these plants were haploids.
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Huang, Q. F., H. Y. Yang. and C. Zhou. 1982. Embryological observations on ovary culture of unpollinated young flowers in Hordeum vulgare L. Acta Botanica Sinica 24: 295-300.
Kharabian, A. and A. Darabi. 2005. Characterization of some chromosomal aberrations in regenerated rice plants (Oryza sativa). Plant cell, Tissue and Organ Culture 83: 161-168.
Kuo, C. S. 1982. The preliminary studies on culture of unfertilized ovary of rice in vitro. Acta Botanica Sinica 24: 33-38.
Toriyama, K., K. Hinata and T. Sasaki. 1986. Haploid and diploid plant regeneration from protoplasts of anther callus in rice. Theoretical and Applied Genetics 73(1): 16-19.
Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 15(3): 473-497.
Raina, S. K. 1989. Tissue culture in rice improvement: Status and potential. Advances in Agronomy 42: 339-397.
Rongbai, L., M. P. Pandey, G. K. Garg, S. K. Pandey, D.K. Dwivedi and Ashima. 1998. Development of a technique for in vitro unpollinated ovary culture in rice, Oryza sativa L.. Euphytica 104: 159-166.
Uchimiya, H., T. Kameya and N. Takanashi. 1971. In vitro culture of unfertilized ovules in Solanum melongena and Zea mays. Japanese Journal of Breeding 21: 247-250.
Yang, H. Y. and C. Zhou. 1982. In vitro induction of haploid plants from unpollinated ovaries and ovules. Theoretical and Applied Genetics 63(2): 97-104.
Zhou, C. and H.Y. Yang. 1980. In vitro induction of haploid plantlets from unpollinated young ovaries of Oryza sativa L.. Acta Genetica Sinica 7: 287-288.
Zhou, C. and H. Y. Yang. 1981a. Induction of haploid rice plantlets by ovary culture. Plant Science Letters 20: 231-237.
Zhou, C. and H. Y. Yang. 1981b. Studies on the in vitro induction of callus from embryo sac of rice. Hereditas 3(5): 10-12.
Zhou, C. and H. Y. Yang. 1981c. In vitro embryogenesis in unfertilized embryo sacs of Oryza sativa L. Acta Botanica Sinica 23: 176-180.
Bindeshwar S. P., R. K. Niroula and H. P. Bimb. 2007. Culture of embryo with a segment of ovary Improved germination and crossability of distant hybrids in indica rice. Scientific World 5 (5): 46-50.
Chen, C., H. Xiao, W. Zhang, A. Wang, Z. Xia, X. Li, W. Zhai, Z. Cheng and Z. Zhu. 2006. Adapting rice anther culture to gene transformation and RNA interferenc. Science in China Series C: Life Sciences 49: 414-428.
Chu, C. C., C. C. Wang, C. S. Sun, C. Hsu, K. C. Yin, C. Y. Chu and F. Y. Bi. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiment on the nitrogen sources. Scientia Sinica 18: 659-668.
Chu, C. C. 1978. The N6 medium and its application to anther culture of cereal crops. pp. 43-50. In: Proceedings of Symposium on Plant Tissue Culture. Science Press, Peking.
Chu, Q. R., H. X. Cao and S. Linscombe. 1977. A novel medium for induction of embryogenic callus in rice anther culture of Southern US crosses. Rice Biotechnology Quarterly 32: 19-20.
De Beauville, A. M. 1980. Obtention haploides in vitro a partir d'ovaires non fecondes de riz, Oryza sativa L. Comptes Rendus Hebdomadaires des Seances de l'Academie des Sciences. Serie D 296: 489-492.
Huang, Q. F., H. Y. Yang. and C. Zhou. 1982. Embryological observations on ovary culture of unpollinated young flowers in Hordeum vulgare L. Acta Botanica Sinica 24: 295-300.
Kharabian, A. and A. Darabi. 2005. Characterization of some chromosomal aberrations in regenerated rice plants (Oryza sativa). Plant cell, Tissue and Organ Culture 83: 161-168.
Kuo, C. S. 1982. The preliminary studies on culture of unfertilized ovary of rice in vitro. Acta Botanica Sinica 24: 33-38.
Toriyama, K., K. Hinata and T. Sasaki. 1986. Haploid and diploid plant regeneration from protoplasts of anther callus in rice. Theoretical and Applied Genetics 73(1): 16-19.
Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 15(3): 473-497.
Raina, S. K. 1989. Tissue culture in rice improvement: Status and potential. Advances in Agronomy 42: 339-397.
Rongbai, L., M. P. Pandey, G. K. Garg, S. K. Pandey, D.K. Dwivedi and Ashima. 1998. Development of a technique for in vitro unpollinated ovary culture in rice, Oryza sativa L.. Euphytica 104: 159-166.
Uchimiya, H., T. Kameya and N. Takanashi. 1971. In vitro culture of unfertilized ovules in Solanum melongena and Zea mays. Japanese Journal of Breeding 21: 247-250.
Yang, H. Y. and C. Zhou. 1982. In vitro induction of haploid plants from unpollinated ovaries and ovules. Theoretical and Applied Genetics 63(2): 97-104.
Zhou, C. and H.Y. Yang. 1980. In vitro induction of haploid plantlets from unpollinated young ovaries of Oryza sativa L.. Acta Genetica Sinica 7: 287-288.
Zhou, C. and H. Y. Yang. 1981a. Induction of haploid rice plantlets by ovary culture. Plant Science Letters 20: 231-237.
Zhou, C. and H. Y. Yang. 1981b. Studies on the in vitro induction of callus from embryo sac of rice. Hereditas 3(5): 10-12.
Zhou, C. and H. Y. Yang. 1981c. In vitro embryogenesis in unfertilized embryo sacs of Oryza sativa L. Acta Botanica Sinica 23: 176-180.
