Aflatoxin B1 Inhibition Using Atoxigenic <I> Aspergillus flavus</I> of Strains
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Abstract
The inhibition of aflatoxin B1 (AFB1) production by toxigenic strain of Aspergillus flavus was examined in the laboratory tests using four of atoxigenic A. flavus strains including A. flavus UPA01 UPA02 UPA03 and UPA04 isolated from maize crop producing areas in Phayao Province, Thailand. The experiment was conducted to find out the colony radial growth rate (Kr) of fungi on solid medium using Potato Dextrose Agar (PDA) and incubated at 30ºC in the dark. The results showed that the Kr values of all A. flavus strains did not appeared significantly difference between A. flavus strains. The average Kr values of all each A. flavus approximately showed as 0.7 cm/day. For the quantities of aflatoxin, A. flavus spore suspension at concentration of 1x105 spore/ml was inoculated in modified yeasted glucose sucrsoe (mYES) at 30ºC in the dark for 7 day. Toxigenic strain of A. flavus NRRL3357 produced and showed AFB1 value as 5.361 ±259.10 ng/ml but all four atoxigenic strains of A. flavus did not produce aflatoxins (AFB1, AFB2, AF1 and AFG2). When A. flavus NRRL3357 was co-incubated with each four atoxigenic A. flavus strains in modified yeasted glucose (mYEG), all atoxigenic A. flavus strains significantly greater inhibited AFB1 from toxigenic A. flavus strain with more seventy percentage of inhibition. The kinetic parameters were expressed by Monod’s equation. The specific growth rates (µ) of all A. flavus were similar. On the other parameter including specific glucose consumption rate (qs), toxigenic strain of A. flavus NRRL3357 was the highest qs value (0.22 mmol/g cell/hr) but it strain was reduced in qs value when co-cultured with atoxigenic strains of A. flavus. The results indicated atoxigenic strains of A. flavus inhibited AFB1 produced by toxigenic strain of A. flavus NRRL3357 with competitive exclusion of substarte (glucose) consumption.
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References
Cotty, P. J. 1988. Aflatoxin and sclerotial production by Aspergillus flavus: Influenceof pH. Phytopathology 78: 1250-1253.
Cotty, P. J. 1994. Influence of field application of an atoxigenic strain of Aspergillus flavus on the population of A. flavus infecting cotton bolls and on the aflatoxin content of cotton seed. Phytopathology 84: 1270-1277.
Cotty, P. J. and P. Bayman. 1993. Completitive exclusion of a toxigenic strain of Aspergillus flavus by an atoxigenic strain. Phytopathology 83: 1283-1287.
Cotty, P. J. and I. J. Misaghi. 1984. Zinniol production by Alternaria sp. Physiology and Biochemistry 74(7): 785-788.
Daigle, D. J. and P. J. Cotty. 1995. Formulating Atoxigenic Aspergillus flavus for field release. Biocontrol Science and Technology 5: 175-184.
Damann, K., R. Sweany and C. DeRobertis. 2004. Frequency of colonization of corn kernels by atoxigenic Aspergillus flavus applied as a potential biocontrol agent. Phytopathology 94: 938-945.
Danmek, K., S. Prasongsuk, P. Lotrakul, K. E. Damann, D. E. Eveleigh and H. Punnapayak. 2011. Effect of Avid® on the synnema-like formation of Aspergillus flavus grown on Czapek medium. African Journal of Microbiology Research 5(18): 2812-2815
Ehrlich, K. C. and P. J. Cotty. 2004. An isolate of Aspergillus flavus used to reduce aflatoxin contamination in cottonseed has a defective polyketide synthase gene. Applied Microbiology and Biotechnology 65: 473-478.
Frandberga, E., J. Pitt and M. Olsena. 2003. Quality control of Aspergillus flavus and A. parasiticus agar and comparison with dichloran 18% glycerol agar: a collaborative study. International Journal of Food Microbiology 89: 99-102.
Glass, N. L. and G. C. Donaldson. 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied Environmental and Microbiology 61(4): 1323-1330.
Ghose, T. K. 1987. Measurement of cellulase activities. Intetntional Union of Pure and Applied Chemistry 59: 257-268.
Horn, B. W., R. L. Greene and J. W. Dorner. 2000. Inhibition of aflatoxin B1 production by Aspergillus parasiticus using nonaflatoxigenic strains: role of vegetative compatibility. Biological Control 17: 147-154.
Klich, M. A. 2007. Environmental and developmental factors influencing aflatoxin production by Aspergillus flavus and Aspergillus parasiticus. Mycoscience 48: 71-80.
Klich, M. A. 2002. Biogeography of Aspergillus species in soil and litter. Mycologia 94(1): 21-27.
Lillehoj, F. B., W. J. Garcia and M. Lambrow. 1974. Aspergillus flavus infection and aflatoxin production in corn: influence of trace elements. Applied Microbiology 28(5): 763-767.
Lopez-Malo, A., S. M. Alazamora and A. Argaiz. 1995. Effect of natural vanillin on germination time and radial growth of moulds in fruit-based agar systems. Food Microbiology 12: 213-219.
Lopez-Malo, A., S. M. Alazamora and A. Argaiz. 1998. Vanillin and pH synergistic effects on mold growth. Journal of Food Science 63(1): 143-146.
Mellon, J. E. and P. J. Cotty. 1995. Expression of elastinolytic activity among isolates in Aspergillus section Flavi. Mycopathologia 131: 115-120.
Miller, G. L. 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analysis of Chemistry 31: 426-428.
Monod, J. 1942. Recherches sur la croissance des Cultures Bactériennes (Thèse 151 Doctorat ès Sciences Naturelles). Hermann, Paris.
Morrison, K. B. and R. C. Righelato. 1974. The relationship between hyphal branching, specific growth rate and colony radial growth rate in Penicillium chrysogenum. Journal of General Microbiology 81(5): 517-520.
Passone, M. A., L. C. Rosso, A. Ciancio, and M. Etcheverry. 2010. Detection and quantification of Aspergillus section Flavi spp. in stored peanuts by real-time PCR of nor-1 gene, and effects of storage conditions on aflatoxin production. International Journal of Food Microbiology 138: 261-281.
Pitt, J. I., A. Hocking and D. R. Glenn. 1983. An improved medium for the detection of Aspergillus flavus and Aspergillus parasiticus. Journal of Applied Bacteriology 54: 109-114.
Ramakrishna, N., J. Lacey and J. E. Smith. 1996. Aspergillus flavus colonization and aflatoxin B1 formation in barley grain during interaction with other fungi. Mycopathologia 136: 53-63.
Reddy, K. N., H. K. Abbas, R. M. Zablotowicz, C. A. Abel and C. H. Koger. 2007. Mycotoxin occurrence and Aspergillus flavus soil propagules in a corn and cotton glyphosate-resistant cropping systems. Food Additives and Contaminants 24(12): 1367-1373.
Reddy, K. R. N., Ch. R. Reddy, P. N. Kumar, C. S. Reddy and Muralidharan. 2009. Genetic variability of aflatoxin B1 producing Aspergillus flavus strains isolated from discolored rice grains. World Journal of Microbiology and Biotechnology 25: 33-39
Rodr´ıguez-Urra, A. B. C. Jimenez, M. Duenas and U. Ugalde. 2009. Bicarbonate gradients modulate growth and colony morphology in Aspergillus nidulans. Federation of European Microbiological Societies 300: 216-221.
Sweany, R. R., K. E. Jr. Damann and M. D. Kaller. 2011. Comparison of soil and corn kernel Aspergillus flavus populations. Phytophatology 101(8): 952-959 p.
Thompson, D. P. 1990. Influence of pH on the fungitoxic activity of naturally occurring compounds. Journal of Food Protection 53: 482-429.
Yoder, J. A., B. S. Christensen, T. J. Croxall, J. L. Tank and D. Sammataro. 2008. Suppression of growth rate of colony-associated fungi by high fructose corn syrup feeding supplement, formic acid, and oxalic acid. Journal of Apicultural Research and Bee World 47(2): 26-130.
