Effect of Brassinosteroid on <I>In Vitro</I> Propagation of <I>Dendrobium delacourii</I>

Abstract

A study on effect of brassinosteroid (Brs) on in vitro propagation of Dendrobium delacouni was carried out by culturing shoots,1 cm in length, onto modified VW media supplemented with 0, 0.01, 0.03, 0.04, 0.05, 0.06, 0.07, 0.08, 0.09, 0.10, 0.15 and 0.20 mg/l Brs. It was found that Brs had no effect on number of leaf, root diameter and number of new shoot. However, 0.08 mg/l Brs induced the best number of roots (9.5 roots) and the best height of new shoots (0.98 cm) while 0.04 mg/l Brs induced the best number of shoots (4.9 shoots). Plants on medium with 0 mg/l Brs produced the lowest number of root.