Effects of 2,4-D and Kinetin on Embryogenic Callus Induction in Rice (<I>Oryza sativa </I>L.) cv. Khao Dawk Mali 105

Abstract

This research aimed to increasing amount of embryogenic callus by using 2,4-D and kinetin. All the experiments were set in Completely Randomized Design (CRD). Rice seeds were cultured on modified Linsmaier and Skoog (LS) medium and supplemented with 0, 2, 4, 6 and 8 mg L^-1 2,4-D. It was found that the modified LS medium supplemented with 2 mg L^-1 2,4-D could induced 67.33% calli which 90.73% were friable calli and 85.79% were greenish-yellow calli after culturing 30 days. Anyhow those calli could not developed into embryoids, after that 2 mg L^-1 2,4-D combined with 2, 4, 6 and 8 mg L^-1 kinetin were applied inorder to increase the production of embryogenic calli. It was found that the modified LS medium supplemented with 2 mg L^-1     2,4-D combined with 2 mg L^-1 kinetin could induced 69.00% calli which 95.64% were friable calli and 89.44% were greenish-yellow calli after culturing 30 days and those calli could developed to embryoids more than using solely 2,4-D or kinetin in every concentration. Therefore the induction percentage, type and color of calli depend on suitable ratio between 2,4-D and kinetin.