Suitable Factors for Establishing Isozyme Patterns in Thai Lady’s Slipper (<I>Paphiopedilum </I>Pfitzer)
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Published:
Sep 3, 2020
Keywords:
Paphiopedilum isozyme electrophoresis
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Abstract
A series of study to find suitable factors for establishing isozyme patterns in 10 Thai Paphiopedilum and 1 natural hybrid showed that 0.5 gram young leaf fresh weight, 0.1 M Tris-HCl pH 7, 1 mM EDTA, 1 % w/v PVP-360, 2 mM DTT, 10 mM β-mercaptoethanol and 11% separating gel are most suitable for polyacrylamide gel electrophoresis (PAGE).
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References
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Al-Jibouri, A.A.M. and K.M. Adham. 1990. Biochemical classification of date palm male cultivars. J. Hort. Sci. 65(6): 725 – 729.
Apavatjrut, P., S. Anuntalabhochai, P. Sirirugsa and C. Alisi. 1999. Molecular markers in the identification of some early flowering Curcuma L. (Zingiberaceae) species. Ann. Bot. 84: 529 – 534.
Byrne, D.H. and T.G. Littleton. 1988. Electrophoresis characterization of diploid plums of the southeastern United States. J. Amer. Soc. Hort. Sci. 113(6): 918 – 924.
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Kumar, S., V.P. Sharma and H.C. Kapoor. 1995. Isozymic identification of cultivars of ber (Zizyphus mauritiana Lamk.). J. Hort. Sci. 70(2): 303 – 306.
Marquard, R.D. 1987. Isozyme inheritance, polymorphism and stability of malate dehydrogenase and phosphoglucose isomerase in Pecan. J. Amer. Soc. Hort. Sci. 112(4): 717 – 721.
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Mowrey, B.D., D.J. Werner and D.H. Byrne. 1990. Inheritance of isocitrate dehydrogenase, malate dehydrogenase, and shikimate dehydrogenase in peach and peach x almond hybrids. J. Amer. Soc. Hort. Sci. 115(2): 312 – 319.
Parfitt, D. E. and S. Arulsekar. 1989. Inheritance and isozyme diversity for GPI and PGM among grape cultivars. J. Amer. Soc. Hort. Sci. 114(3): 486 – 491.
Sotis, D. E., A. J. Gilmartin, L. Rieseberg and S. Gardner. 1987. Genetic variation in the epiphytes Tillandsia ionantha and T. recurvata (Bromeliaceae). Amer. J. Bot. 74(4):
531-537.
อบฉันท์ ไทยทอง. 2544. กล้วยไม้เมืองไทย, พิมพ์ครั้งที่ 2. บ้านและสวน, กรุงเทพฯ. 461 น.
Al-Jibouri, A.A.M. and K.M. Adham. 1990. Biochemical classification of date palm male cultivars. J. Hort. Sci. 65(6): 725 – 729.
Apavatjrut, P., S. Anuntalabhochai, P. Sirirugsa and C. Alisi. 1999. Molecular markers in the identification of some early flowering Curcuma L. (Zingiberaceae) species. Ann. Bot. 84: 529 – 534.
Byrne, D.H. and T.G. Littleton. 1988. Electrophoresis characterization of diploid plums of the southeastern United States. J. Amer. Soc. Hort. Sci. 113(6): 918 – 924.
Durham, R.E., G.A. Moore and W.B. Sherman. 1987. Isozyme banding patterns and their usefulness as genetic markers in peach. J. Amer. Soc. 112(6): 1013 – 1018.
Kumar, S., V.P. Sharma and H.C. Kapoor. 1995. Isozymic identification of cultivars of ber (Zizyphus mauritiana Lamk.). J. Hort. Sci. 70(2): 303 – 306.
Marquard, R.D. 1987. Isozyme inheritance, polymorphism and stability of malate dehydrogenase and phosphoglucose isomerase in Pecan. J. Amer. Soc. Hort. Sci. 112(4): 717 – 721.
Moore, G.A. and G.B. Collins. 1983. New challenges confronting plant breeders. p. 25 - 59. In S.D. Tanksley and T.J. Orton (eds.). Isozyme in Plant Genetics and Breeding, Part A. Elsevier Science Publishers B. V., Amsterdam.
Mowrey, B.D., D.J. Werner and D.H. Byrne. 1990. Inheritance of isocitrate dehydrogenase, malate dehydrogenase, and shikimate dehydrogenase in peach and peach x almond hybrids. J. Amer. Soc. Hort. Sci. 115(2): 312 – 319.
Parfitt, D. E. and S. Arulsekar. 1989. Inheritance and isozyme diversity for GPI and PGM among grape cultivars. J. Amer. Soc. Hort. Sci. 114(3): 486 – 491.
Sotis, D. E., A. J. Gilmartin, L. Rieseberg and S. Gardner. 1987. Genetic variation in the epiphytes Tillandsia ionantha and T. recurvata (Bromeliaceae). Amer. J. Bot. 74(4):
531-537.
