Comparison between 2 Types of Media on Growth and Development of Arabica Coffee Leaf Tissue
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Published:
Oct 9, 2020
Keywords:
arabica coffee leaf culture tissue culture medium
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Abstract
Leaf explants of Populacao 5 arabica coffee were cultured on 2 types of media namely: Sondahl and Sharp (1977) (SS) and Kahia and Owuor (1990) (KO) with some adjustments. Rate of survival, callus production, amount of callus and embryo production during 50 days, 70 days, 8 months and 16 months were estimated. Explants cultured on KO medium showed higher rate of survival, amount of proliferating callus and rate of somato embryonic production while SS medium had lower rate of those aspects but higher deformation of somatic embryos. All survived explants produced calli on both medium. Supplement of coconut liquid endosperm did not show significant effect on development of somatic embryos.
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References
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Sharp, W.R., L.S. Caldas, L.C. Monaco and A. Carvalho, 1973. Production of Coffea arabica callus of three ploidy levels and subsequent morphogenesis. Phyton 31: 67-74.
Starisky, G. 1970. Embryoid formation in callus cultures of coffee. Acta Bot. Neerl. 19: 509-514.
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Sondahl, S., T. Nakamura, H.P. Medina-Filho, A. Carvalho, L.C. Fazuoli and W.M. Costa. 1983. Coffee. In: Handbook of Plant Cell Culture, Vol.1, Techniques for Propagation and Breeding. D.A. Evans, W.R. Sharp, P.V. Ammirato and Y. Yamada (editors). Macmillan Publishing Company, New York. pp. 564-590.
นิธิ ไทยสันทัด. 2540. อิทธิพลของพันธุ์ และเส้นกลางใบต่อการสร้างแคลลัสในการเพาะเลี้ยงเนื้อเยื่อใบกาแฟ อราบิก้า (Coffed arabica L.). วารสารเกษตร 13 (1): 43-51.
Herman, F.R.P. and G.J. Haas. 1975. Clonal propagation of Coffea arabica L. from callus culture. Hort. Sci. 10: 588-589.
Kahia, J. W. and J.B.O. Owuor. 1990. In vitro propagation of disease resisitant Coffea arabica L. cultivar-Ruiru 11. Kenya Coffee 55(646): 901-905.
Kartha, K.K., K.P. Mroginski and N.L. Lueng. 1981. Germplasm preservation of coffee (Coffea arabica L.) by in vitro culture of shoot apical meristems. Plant Sci. Lett. 22: 301-307.
Linmaier, M. and F. Skoog. 1965. Organic growth factor requirements of tobacco tissue cultures. Physiol. of coffee. Acta Bot. Neerl. 19: 509-514.
Murashige, T. and F. Skoog. 1962. A medium for rapid growth and bioassay with tobacco tissue culture. Physiol. Plant. 15: 473-479.
Op de Laak, J.H.M. 1992. Arabica Coffee Cultivation and Extension Manual for the Highlands of Northern Thailand. Thanaban Printer, Chiangmai. 111p.
Sharp, W.R., L.S. Caldas, L.C. Monaco and A. Carvalho, 1973. Production of Coffea arabica callus of three ploidy levels and subsequent morphogenesis. Phyton 31: 67-74.
Starisky, G. 1970. Embryoid formation in callus cultures of coffee. Acta Bot. Neerl. 19: 509-514.
Sondahl, S. and W.R. Sharp. 1977. High frequency induction of somatic embryos in cultured leaf explants of Coffea arabica L. Z. Pflanzenphysiol. Bd. 81:(s) 395-408.
Sondahl, S., T. Nakamura, H.P. Medina-Filho, A. Carvalho, L.C. Fazuoli and W.M. Costa. 1983. Coffee. In: Handbook of Plant Cell Culture, Vol.1, Techniques for Propagation and Breeding. D.A. Evans, W.R. Sharp, P.V. Ammirato and Y. Yamada (editors). Macmillan Publishing Company, New York. pp. 564-590.
