Identification of Longan Varieties by Electrophoretic Method

Abstract

Identification was carried out on sixteen varieties and eight Daw clones of longan (Euphoria longana Lam.). These are Puenmuang Lumphun, Kradook, Chompoo Nam, Chompoo, Soitong, Baihod, Biewkiew Chiang Mai, Daengklom, Nampueng, Biewkiew Pasao, Haew, Puangtong, E-lueong, Kiew Pra In, Baidam, Puenmuang Nan, Daw Donchai, Daw Yoddaeng, Daw Yodkao, Daw Hom, Daw Kankaeng, Daw Kan-on, Daw Nan, Daw Tanoi.

            Electrophoretic method was used to determine isozyme patterns from mature leaves, using 0.05 M Tris-HCI buffer, pH 8.4 (150 mM NaCl, 10 mM cysteine, 1 mM ascorbic acid, 1 mM Cacl₂, ImM Na₂ EDTA, 2% nicotine). Polyacrylamide vertical slab gel electrophoresis was used at 8.5 per cent for peroxidase and 10 percent for acid phosphatase and esterase separation. It was found that sixteen longan varieties could be identified by peroxidase, acid phosphatase and esterase while eight Daw clones could be identified by peroxidase and acid phosphatase. Isozyme analysis showed banding patterns of 10, 10 and 3 bands for peroxidase, acid phosphatase and esterase, respectively.