Improvement Postpartum Reproductive Efficiency of Crossbred Native x Holstein Dairy Cows through Active Immunization against Testosterone
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Oct 15, 2020
Keywords:
immunization active Testosterone Progesterone dairy cow
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Abstract
The objective of this study was to immunize crossbred dairy cows from small holder village farms against Testosterone to improve efficiency of postpartum reproduction. Immunogen was Testosterone-3-(O-Carboxymethyl)Oxime-Human Serum Albumin (T4-HSA). Experimental animals were from village farms in Mae Rim, Sun Sai and Sun Kumpaeng district of Chiang Mai province and Ban-Ti district of Lumphun province. Most cows were slow to conceive after artificial insemination. Experimental design was Completely Randomized Design (CRD) with regiment: control and actively immunized against Testosterone (Anti-T4). The cows were immunized two times with 1 month apart. Milk samples were collected twice weekly and terminated until 135 days postimmunization. Body condition score (BCS) between 1 to 5 were also recorded at the time of immunization. The results of this study were that : cows from within control and Anti-T4 had area under postpartum progesterone concentration 6.2±1.3 and 8.2±1.1 units; body condition score 3.3±0.1 and 2.9±0.1 (P<.05); pregnancy rate 63.6 and 77.8% (P<.05), respectively. These study demonstrated that immunization against Testosterone can be use for improvement reproductive efficiency of dairy cows under farm condition, especially with slow to conceive cows in small holder village farms.
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มณีวรรณ กมลพัฒนะ, กิติยา ศรีศักดิ์วัฒนะและ โสภณ สรรเพชญ. 2531. การตรวจท้องโคนมระบบบริการ และผลงาน. ใน : บทบาทการตรวจท้องโคนม จากน้ำนม. หน้า 73-126. โรงพิมพ์จุฬาลงกรณ์ มหาวิทยาลัย, กรุงเทพฯ.
เพทาย พงษ์เพียจันทร์ และทัศนีย์ อภิชาติสรางกูร. 2533. การประเมินประสิทธิภาพการสืบพันธุ์ของโคนมโดย วิธีเรดิโออิมมูนโนแอสเซ. วารสารเกษตร 6(1): 21-40.
อภิชาติ รัตนวนิช, เพทาย พงษ์เพียจันทร์, พันทิพา พงษ์เพียจันทร, ชาตรี ทองอินทร์ และวิชาญ สุขประเสริฐ. 2542. ผลการกระตุ้นภูมิคุ้มกันแบบพาสซีฟต้านฮอร์โมนเทสทอสเตอร์โรน ต่อการทำงานของรังไข่หลังคลอดของแม่โคนมลูกผสมพื้นเมือง x โฮลสไตน์. วารสารเกษตร 14(2): 189 198.
Apichartsrungkoon, T. and P. Pongpiachan. 1990. Use of milk progesterone profile, for the study of fertility in the postpartum period of dairy cow. J. Agriculture 6(3): 219-225.
Armstrong, R.W., J. Gauldie and E.V. Younglai. 1978 Effects of active immunization of female rabbits against testosterone. J. Endocr. 79: 339-347.
Bourtourault, M., V. Shacoori, J. Guerin, B. Saiag and Rault. 1991. Research Communications in Chemical Pathology and Pharmacology 72(3): 273-284.
Cox, R.I., P.A. Wilson, R.J. Scaramuzzi, R.M. Hoskinson, J.M. George and B.M. Bindon. 1982. The active immunization of sheep against oestrone, androstenedione or testosterone to increase twinning. Proc. Aust. Soc. Anim. Prod. 14: 511-514
Dobson, H. 1987. Effect of transport stress on luteinizing hormone released by GnRH in dairy cows. Acta Endocrinologica (Copenh.) 115: 63-66.
D'Occhio, M.J., D.R. Gifford, R.M. Hoskinson, T. Weatherly, P.F. Flavel, R.E. Mattner and B.P. Setchell. 1987. Reproductive hormone secretion and testicular growth in bull calve active immunized against testosterone and oestradiol-17β. J. Reprod. Fertil. 79(2): 315-324.
Hillier, S.G., G.V. Groom, A.R. Boyns and E.H.D. Cameron. 1974. Development of polycystic ovaries in rats actively immunized against T-3-BSA. Nature 250: 433-434.
Kamonpatana, M., V. Timsard, C. Pansin and R.I. Cox, 1985. Steroid immunization in the swamp buffalo: Potential for subfertility recovery. Buffalo J. 1: 53-60.
Lermite, V., J. Thimonier, R. Dufour and M. Terqui. 1991. Effect of the increase of steroid binding plasma levels after passive immunization against testosterone on the control of luteinizing hormone (LH) secretion in ovariectomized underfed dairy heifers. Reprod. Nutr. Dev. 31(5): 541-549.
Lermite, V., L. Delaby, J. Thimonier, R. Dufour and N. Terqui. 1993. Effect of passive immunization against testosterone on reproductive hormone secretion and ovarian function in dairy cows and pubertal beef heifers. Theriogenology 39(2): 507-526.
Martensz, N.D. and R.J. Scaramuzzi. 1979. Plasma concentration of luteinizing hormone, follicle stimulating hormone and progesterone during the breeding season in ewes immunized against androstenedione or testosterone. J. Endocr. 81: 249-259.
Nieschlag, E., K. Usadel, U. Schwedes, H.K. Kley, K. Schoffling and H.L. Kruskemper. 1973. Alteration in the testicular morphology and function in rabbits following active immunization with testoster-one. Endocrinology 92: 1142-1147.
Nieschlag, E., H.K. Usadel, H.K. Kley, U. Schwedes, K. Schoffling and H.L. Kruskemper. 1974. A new approach for investigating hypothalamo-pituitary-gonadal and adrenal feedback control mechanism: Active immunization with steroids. Acta Endocrinol. 76: 556-569.
Price, C.A., B.A. Morris and R. Webb. 1987. Reproductive and endocrine effects of active immunization against testosterone conjugate in the heifer. J. Reprod. Fert. 81: 149-160.
Rhind, S.M., R.G. Gunn, B.A. Morris, J. Clayton, I.D. Leslie and G. Gittus. 1985. Effect of passive immunization against testosterone on the reproductive performance of Scottish Blackface ewes in different levels of body condition at mating. Anim. Prod. 41: 97-102.
Schanbacher, B.D. and J.J. Ford. 1977. Gonadotropin secretion in cryptorchid and castrate rams and the acute effects of exogenous steroid treatment. Endocrinology 100: 387-393.
Steel, R.G.D. and J.H. Torrie. 1962. Principle and Proceures of Statistics. McGraw-Hill Book Co. Inc., New York.
Stoebel, D.P. and G.P. Moberg. 1979. Effect of ACTH and cortisol on estrus behavior and the luteinizing hormone surge in the cow. Fed. Proc. 38: 1254.
Thompson, Jr., D.L., L.L Southern, St. R.L. George, L.S. Johnes and Jr. F. Garza. 1985. Active immunization of prepubertal boars against testosterone: Testicular and endocrine responses at 14 months of age. J. Anim. Sci. 61(6): 1498-1504.
Walker, M.P., Jr. D.L. Thompson, R.A. Godke and P.G. Honey. 1984. Active immunization of prepubertal bulls against testosterone: Seminal and testicular characteristics after puberty. Theriogenology 22: 269-278.
Wildman, E.E., G.M. Jones, P.E. Wagner, R.L. Boman H.F. Troutt and H.F. Lesch. 1982. A dairy cow body condition scoring system and its relationship to selected production characteristics. J. Dairy Sci. 65: 495-501.
Zarrow, M.X., J.M. Yochim, J.L. McCarthy and R.C. Sanborn. 1964. Experimental Endocrinology: A Textbook of Basic Techniques. Academic Press, New York.
เพทาย พงษ์เพียจันทร์ และทัศนีย์ อภิชาติสรางกูร. 2533. การประเมินประสิทธิภาพการสืบพันธุ์ของโคนมโดย วิธีเรดิโออิมมูนโนแอสเซ. วารสารเกษตร 6(1): 21-40.
อภิชาติ รัตนวนิช, เพทาย พงษ์เพียจันทร์, พันทิพา พงษ์เพียจันทร, ชาตรี ทองอินทร์ และวิชาญ สุขประเสริฐ. 2542. ผลการกระตุ้นภูมิคุ้มกันแบบพาสซีฟต้านฮอร์โมนเทสทอสเตอร์โรน ต่อการทำงานของรังไข่หลังคลอดของแม่โคนมลูกผสมพื้นเมือง x โฮลสไตน์. วารสารเกษตร 14(2): 189 198.
Apichartsrungkoon, T. and P. Pongpiachan. 1990. Use of milk progesterone profile, for the study of fertility in the postpartum period of dairy cow. J. Agriculture 6(3): 219-225.
Armstrong, R.W., J. Gauldie and E.V. Younglai. 1978 Effects of active immunization of female rabbits against testosterone. J. Endocr. 79: 339-347.
Bourtourault, M., V. Shacoori, J. Guerin, B. Saiag and Rault. 1991. Research Communications in Chemical Pathology and Pharmacology 72(3): 273-284.
Cox, R.I., P.A. Wilson, R.J. Scaramuzzi, R.M. Hoskinson, J.M. George and B.M. Bindon. 1982. The active immunization of sheep against oestrone, androstenedione or testosterone to increase twinning. Proc. Aust. Soc. Anim. Prod. 14: 511-514
Dobson, H. 1987. Effect of transport stress on luteinizing hormone released by GnRH in dairy cows. Acta Endocrinologica (Copenh.) 115: 63-66.
D'Occhio, M.J., D.R. Gifford, R.M. Hoskinson, T. Weatherly, P.F. Flavel, R.E. Mattner and B.P. Setchell. 1987. Reproductive hormone secretion and testicular growth in bull calve active immunized against testosterone and oestradiol-17β. J. Reprod. Fertil. 79(2): 315-324.
Hillier, S.G., G.V. Groom, A.R. Boyns and E.H.D. Cameron. 1974. Development of polycystic ovaries in rats actively immunized against T-3-BSA. Nature 250: 433-434.
Kamonpatana, M., V. Timsard, C. Pansin and R.I. Cox, 1985. Steroid immunization in the swamp buffalo: Potential for subfertility recovery. Buffalo J. 1: 53-60.
Lermite, V., J. Thimonier, R. Dufour and M. Terqui. 1991. Effect of the increase of steroid binding plasma levels after passive immunization against testosterone on the control of luteinizing hormone (LH) secretion in ovariectomized underfed dairy heifers. Reprod. Nutr. Dev. 31(5): 541-549.
Lermite, V., L. Delaby, J. Thimonier, R. Dufour and N. Terqui. 1993. Effect of passive immunization against testosterone on reproductive hormone secretion and ovarian function in dairy cows and pubertal beef heifers. Theriogenology 39(2): 507-526.
Martensz, N.D. and R.J. Scaramuzzi. 1979. Plasma concentration of luteinizing hormone, follicle stimulating hormone and progesterone during the breeding season in ewes immunized against androstenedione or testosterone. J. Endocr. 81: 249-259.
Nieschlag, E., K. Usadel, U. Schwedes, H.K. Kley, K. Schoffling and H.L. Kruskemper. 1973. Alteration in the testicular morphology and function in rabbits following active immunization with testoster-one. Endocrinology 92: 1142-1147.
Nieschlag, E., H.K. Usadel, H.K. Kley, U. Schwedes, K. Schoffling and H.L. Kruskemper. 1974. A new approach for investigating hypothalamo-pituitary-gonadal and adrenal feedback control mechanism: Active immunization with steroids. Acta Endocrinol. 76: 556-569.
Price, C.A., B.A. Morris and R. Webb. 1987. Reproductive and endocrine effects of active immunization against testosterone conjugate in the heifer. J. Reprod. Fert. 81: 149-160.
Rhind, S.M., R.G. Gunn, B.A. Morris, J. Clayton, I.D. Leslie and G. Gittus. 1985. Effect of passive immunization against testosterone on the reproductive performance of Scottish Blackface ewes in different levels of body condition at mating. Anim. Prod. 41: 97-102.
Schanbacher, B.D. and J.J. Ford. 1977. Gonadotropin secretion in cryptorchid and castrate rams and the acute effects of exogenous steroid treatment. Endocrinology 100: 387-393.
Steel, R.G.D. and J.H. Torrie. 1962. Principle and Proceures of Statistics. McGraw-Hill Book Co. Inc., New York.
Stoebel, D.P. and G.P. Moberg. 1979. Effect of ACTH and cortisol on estrus behavior and the luteinizing hormone surge in the cow. Fed. Proc. 38: 1254.
Thompson, Jr., D.L., L.L Southern, St. R.L. George, L.S. Johnes and Jr. F. Garza. 1985. Active immunization of prepubertal boars against testosterone: Testicular and endocrine responses at 14 months of age. J. Anim. Sci. 61(6): 1498-1504.
Walker, M.P., Jr. D.L. Thompson, R.A. Godke and P.G. Honey. 1984. Active immunization of prepubertal bulls against testosterone: Seminal and testicular characteristics after puberty. Theriogenology 22: 269-278.
Wildman, E.E., G.M. Jones, P.E. Wagner, R.L. Boman H.F. Troutt and H.F. Lesch. 1982. A dairy cow body condition scoring system and its relationship to selected production characteristics. J. Dairy Sci. 65: 495-501.
Zarrow, M.X., J.M. Yochim, J.L. McCarthy and R.C. Sanborn. 1964. Experimental Endocrinology: A Textbook of Basic Techniques. Academic Press, New York.
