Effects of Cultivar and Midvein on Callus Formation of Arabica Coffee (<I>Coffea arabica</I> L.) Leaf Culture
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Abstract
Leaf culture of Catimor arabica coffee was carried out to study effects of cultivars and existence of midvein on production of callus tissue. It was found that among 3 lines studied, C1669-31, Progeny90 and Populacao5; the latest showed highest rate of callus production and amount of callus whether on explants with or without midvein. Explants with midvein produced better result in amount of callus proliferation, except in Progeny90 of which no difference was found. Percentage of callus production was lower in explants with midvein than those without midvein due to higher contamination rate. Somato-embryonic developinent was failed to succeed. All callus turned brown and finally disintegrated without further development within 15 weeks on SS2 medium.
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References
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