Selection of <I>Pleurotus ostreatus</I> [(Jacq.ex.Fr) Kummer] Monokaryons for Hybridization

Abstract

Forty two monokaryons, grown from single spore of the oyster mushroom, were divided into 4 groups of very fast, fast, slow and very slow mycelial growth rates. Five monokaryons were selected into each group, and all combinations of intercrossing between them were made. A total of 40 combinations were found to have clamp connections of which only 24 produced fruit bodies. In this study, good capability of having clamp connection 'and fruit bodies formations were obtaind from the interbreeding between the first three fast mycelial growth rate. Breeding among the same group or with the lower mycelial growth rate failed to produce neither clamp connection nor satisfied yield. Potential yield of the dikaryotic hybrids can neither be indicated from the zymogram of isozyme of esterase, acid phosphatase, nor total proteins of the monokaryons. Identification of the origin of the dikaryons from any monokaryons combination was not possible.