Selecting Diploid Potato Clones Suitable for Transposon Tagging of the R-genes against Late Blight (<I>Phytophthora bare infestans</I>): Selecting Genotypes Carrying R1 and R3
Article Sidebar
เผยแพร่แล้ว:
ต.ค. 27, 2020
คำสำคัญ:
-
Main Article Content
บทคัดย่อ
The process of developing and selecting diploid potato (Solanum tuberosum L.) clones for transposon tagging of the Phytophthora infestans resistance genes-R, was applied to various potato genotypes. The plants carrying allele R1 or R3 were selected for possessing the following characteristics: diploid genome, high fertility, straight segregation of the R-allele, efficicient regeneration in vitro and high rate of transformation by the Agrobacterium tumefaciens carrying genes NPTII and GUS interon. Three of each R1-and R3 -carrying clones performed those desirable characteristics.
Article Details
บท
บทความวิจัย
References
Draper, J., Scott, R., Armitage, P., and Walden, R. dopo (1988). Plant genetic transformation and gene expression: a laboratory mannual. pp. 315-327.
Hoekema, A., Hirsch, P.R., Hooykaas, P.J.J., and mbo Schilperoort, R.A. (1983) A binary plant vector strategy based on seperation of vir-and T-region re of the Agrobacterium tumefaciens T plasmid. Nature 303: 179-180.
Hougas, R.W. and Peloquin, S.J. (1958) The potential of potato haploids in breeding and genetic research. American Potato Journal 35: 701-711.
Hutten, R.C.B., Scholberg, EJ.M.M. , and Hermsen, J.G.Th. (1990). Analysis of dihaploid production and induction ability and tetraploid X pollinator in teraction (abstract). 11th Triennial
Conference EAPAR, Edinbergh, U.K. pp.117-118.
Jacob, J.P. and Yoder, I.P. (1989). Ploidy levels in transgenic tomato plants determined by choroplast number Plant Cell Reports 7 (8): 662-664
Jefferson, R.A., Kavanagh, T.A. and Bevan, M.W. (1987). GUS fusion: B-glucuronidase as a sensitive and versatile gene fusion marker in higher plant. EMBO Journal of Environmental Horticulture 9(1): 51-56.
Lapwood, D.H. (1961). Laboratory assessments of the susceptibility of potato haulm to blight (Phytophthora infestans). European Potato Journal 4(2): 116-128.
McClintock, B. (1945). Cytogenetic studies of maize and Neurospora. In: Moore, J.A. (ed.) (1987). The discovery and characterization of transposable elements: the collected papers of Barbara McClintock. Garland Publishing Inc. New York
Mooi, J.C. (1965). Experiments on testing field resistance to Phytophthora infestans by inoculating cut leaves of potato varieties. European Potato Journal 8(3): 182-183.
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant 15: 463-497.
Neal, C.E. and Topoleski, L.D. (1983). Effect of the basal medium on the development of tomato embryos in intro. Journal of American Society of Horticultural Science 110: 434-438.
Old, R.W. and Primrose, S.B. (1989). Principle of Gene Manipulation and Introduction to Genetic Engineering. 4th ed., Oxford. 438 p.
Singsit, C. and Hanneman, R.E. (1991). Rescuing abortive inter-EBN potato Hibrids through double pollination and embryo culture. Plant Cell Reports 9: 475-478.
Thaisantad, N. (1992) .Selecting diploid potato clones suitable for transposon tagging of the Rgenes against late blight (Phytophthora infestans), MS thesis, Wageningen Agricultural University, Wageningen, the Netherlands, 64 p.
Toxopeus, H.J. (1954). Leaf testing as a method of on genetical analysis of immunity from Phytophthora infestans in potatoes. Euphytica 3: 233-240.
Umaerus, V. (1969a) [Studies on field resistance to Phytophthora infestans 1. The infection efficiency of zoospores of P. infestans as influenced by the host genotype]. Z. PfIZuchl. 19: 91-107.
Umaerus, V. (1969b) Studies on field resistance to Phytophthora infestans 2. A method of screening young potato seedlings for field resistance to P. infestans. Z.PflaZuchl 61: 167-194.
Umaerus, V and Lihnell, D. (1976) A laboratory method for measuring the degree of attack by Phytophthora infestans Potoato Research 19: 91-107.
Vancaneyt, G., Schmidt, R. O'Conor-Sanchez, A. Wilmitzer, L. and Rocha-Sosa, M. (1990). Construction of an interon-containing marker gene: splicing of the interon in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation. Molecular and Applied Genetics 220: 245-250.
Visser, R.F. (1989). Manipulation of the starch composition of Solanum tuberosum L. using Agrobacterium mediated transformation. Ph.D. Thesis. University of Groningen, the Neth- erlands.
Visser, R.F. (1991). Regeneration and transformation of potato by Agrobacterium tato by Agro terium tumefaciens In: Lindsey, K. (ed.) (1991) Plant Tissue Cul ture Mannual: Fundamental and Applications. Dordrecht, Klumer. B5: 1-9.
Visser, R.F., Jacobsen, E., Hesseling-Meinders, A., Schans, M.J., Wilthout, B., Feenstra, W.J.
(1989a). Transformation of homozygous diploid potato with an Agrobacterium tumefaciens binary vector system by adventitious root regeneration on leaf and stem explants. Plant Molecular Biology 12: 329-337.
Visser, R.F., Jacobsen, E., Wilthout, B., and Feentra W.J. (1989b). Efficient transformation of potato (Solanum tuberosum L.) using binary vector in Agrobacterium rhizogenes. Theoritical Applied Genetics 78: 594-600.
Hoekema, A., Hirsch, P.R., Hooykaas, P.J.J., and mbo Schilperoort, R.A. (1983) A binary plant vector strategy based on seperation of vir-and T-region re of the Agrobacterium tumefaciens T plasmid. Nature 303: 179-180.
Hougas, R.W. and Peloquin, S.J. (1958) The potential of potato haploids in breeding and genetic research. American Potato Journal 35: 701-711.
Hutten, R.C.B., Scholberg, EJ.M.M. , and Hermsen, J.G.Th. (1990). Analysis of dihaploid production and induction ability and tetraploid X pollinator in teraction (abstract). 11th Triennial
Conference EAPAR, Edinbergh, U.K. pp.117-118.
Jacob, J.P. and Yoder, I.P. (1989). Ploidy levels in transgenic tomato plants determined by choroplast number Plant Cell Reports 7 (8): 662-664
Jefferson, R.A., Kavanagh, T.A. and Bevan, M.W. (1987). GUS fusion: B-glucuronidase as a sensitive and versatile gene fusion marker in higher plant. EMBO Journal of Environmental Horticulture 9(1): 51-56.
Lapwood, D.H. (1961). Laboratory assessments of the susceptibility of potato haulm to blight (Phytophthora infestans). European Potato Journal 4(2): 116-128.
McClintock, B. (1945). Cytogenetic studies of maize and Neurospora. In: Moore, J.A. (ed.) (1987). The discovery and characterization of transposable elements: the collected papers of Barbara McClintock. Garland Publishing Inc. New York
Mooi, J.C. (1965). Experiments on testing field resistance to Phytophthora infestans by inoculating cut leaves of potato varieties. European Potato Journal 8(3): 182-183.
Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant 15: 463-497.
Neal, C.E. and Topoleski, L.D. (1983). Effect of the basal medium on the development of tomato embryos in intro. Journal of American Society of Horticultural Science 110: 434-438.
Old, R.W. and Primrose, S.B. (1989). Principle of Gene Manipulation and Introduction to Genetic Engineering. 4th ed., Oxford. 438 p.
Singsit, C. and Hanneman, R.E. (1991). Rescuing abortive inter-EBN potato Hibrids through double pollination and embryo culture. Plant Cell Reports 9: 475-478.
Thaisantad, N. (1992) .Selecting diploid potato clones suitable for transposon tagging of the Rgenes against late blight (Phytophthora infestans), MS thesis, Wageningen Agricultural University, Wageningen, the Netherlands, 64 p.
Toxopeus, H.J. (1954). Leaf testing as a method of on genetical analysis of immunity from Phytophthora infestans in potatoes. Euphytica 3: 233-240.
Umaerus, V. (1969a) [Studies on field resistance to Phytophthora infestans 1. The infection efficiency of zoospores of P. infestans as influenced by the host genotype]. Z. PfIZuchl. 19: 91-107.
Umaerus, V. (1969b) Studies on field resistance to Phytophthora infestans 2. A method of screening young potato seedlings for field resistance to P. infestans. Z.PflaZuchl 61: 167-194.
Umaerus, V and Lihnell, D. (1976) A laboratory method for measuring the degree of attack by Phytophthora infestans Potoato Research 19: 91-107.
Vancaneyt, G., Schmidt, R. O'Conor-Sanchez, A. Wilmitzer, L. and Rocha-Sosa, M. (1990). Construction of an interon-containing marker gene: splicing of the interon in transgenic plants and its use in monitoring early events in Agrobacterium-mediated plant transformation. Molecular and Applied Genetics 220: 245-250.
Visser, R.F. (1989). Manipulation of the starch composition of Solanum tuberosum L. using Agrobacterium mediated transformation. Ph.D. Thesis. University of Groningen, the Neth- erlands.
Visser, R.F. (1991). Regeneration and transformation of potato by Agrobacterium tato by Agro terium tumefaciens In: Lindsey, K. (ed.) (1991) Plant Tissue Cul ture Mannual: Fundamental and Applications. Dordrecht, Klumer. B5: 1-9.
Visser, R.F., Jacobsen, E., Hesseling-Meinders, A., Schans, M.J., Wilthout, B., Feenstra, W.J.
(1989a). Transformation of homozygous diploid potato with an Agrobacterium tumefaciens binary vector system by adventitious root regeneration on leaf and stem explants. Plant Molecular Biology 12: 329-337.
Visser, R.F., Jacobsen, E., Wilthout, B., and Feentra W.J. (1989b). Efficient transformation of potato (Solanum tuberosum L.) using binary vector in Agrobacterium rhizogenes. Theoritical Applied Genetics 78: 594-600.