PRODUCTION OF VIRUS-FREE CHRYSANTHEMUM BY USING THE COMBINATION OF HEAT TREATMENT AND MERISTEM CULTURE
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Abstract
Chrysanthemum (Chrysanthemum morifolium) cv. Seiko-no-hana, which showed mosaic, mottle, veinalchlorosis, flower distortion and irregular blooming symptoms from the virus infection, were kept at 37 + 2 C. for 1, 2, 3, 4, 5, and 6 weeks respectively before the meristems were cut and cultured on the modified Murashige and Skoog medium (1962) containing 0.4 ppm BAP and 0.05 ppm IAA. Plantlets about 3-4 cm. size with good root system were transferred in the sterilized soil mixture and tested for the viruses by using both indicator plants (Chenopodium amaranticolor and Nicotiana glutinosa) and electronmicroscopy. Chrysanthemum Virus B and other virus which could not yet be clearly identified as Tomato Aspermy Virus were found.
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References
Asatani, M. (1972). Freeing chrysanthemum from the rod shaped leaf mottling viruses and tomato aspermy virus by a combination meristem-tip culture with heat treat ment. Ber. Oharo. Inst. Landwirthsh. Biol. 15: 169-179.
Gibbs, A. J. (1979). Cucumber mosaic virus. CMI / AAB Description of Plant Viruses. No. 213.
Hakkart, F. A. and Quak, F. (1964). Effect of heat treatment of young plants on freeing chrysanthemum from virus B by means of meristems culture. Neth. J. Plant Path. 70: 154-170.
Hollings, M. and Stone, O.M. (1970). Attempts to eliminate chrysanthemum stunt from chrysanthemum by meristem-tip culture after heat treatment. Am. Appl. Biol. 65: 311-315.
Hollings, M. and Stone, O.M. (1971). Tomato aspermy virus. CMI / AAB Description of Plant Viruses. No. 79
Hollings, M. and Stone, O.M. (1972). Chrysanthemum virus B. CMI / AAB Description of Plant Viruses. No. 110.
Mckay, M. E. (1977). Growing Chrysanthemum, Cut Flower and Pot Plants. Horticul ture Branch Advisory Leaflet, H 30. Queensland Department of Primary Indus tries, 14 p.
Moldgate, D. P. (1977). Propagation of ornamentals by tissue culture. In: Plant Cell. Tissue and Organ Culture, edited by J. Reinert and Y. P. S. Bajaj. Springer Verlag, Berlin. p 44-65.
Pouldan, N. (1963). Virus-free plants of chrysanthemum from approved varieties established by means of heat treatment and meristem-tip culture. Tiddskr Plan teavl 77: 689-696.
Quak, F. (1977). Meristem Culture and Virus-free Plant. In: Plant Cell, Tissue and Organ Culture, edited by J. Reinert and Y. P. S. Bajaj. Springer Verlag, Berlin. p. 598 615.
Walkey, D. G. A. (1980). Production of Virus-free Plants by Tissue Culture. In: Tissue Culture Methods for Plant Pathologists, edited by D. S. Ingram and J. P. Helgeson. Blackwell Scientifics Publications, Oxford. p. 109-117. Insert
Gibbs, A. J. (1979). Cucumber mosaic virus. CMI / AAB Description of Plant Viruses. No. 213.
Hakkart, F. A. and Quak, F. (1964). Effect of heat treatment of young plants on freeing chrysanthemum from virus B by means of meristems culture. Neth. J. Plant Path. 70: 154-170.
Hollings, M. and Stone, O.M. (1970). Attempts to eliminate chrysanthemum stunt from chrysanthemum by meristem-tip culture after heat treatment. Am. Appl. Biol. 65: 311-315.
Hollings, M. and Stone, O.M. (1971). Tomato aspermy virus. CMI / AAB Description of Plant Viruses. No. 79
Hollings, M. and Stone, O.M. (1972). Chrysanthemum virus B. CMI / AAB Description of Plant Viruses. No. 110.
Mckay, M. E. (1977). Growing Chrysanthemum, Cut Flower and Pot Plants. Horticul ture Branch Advisory Leaflet, H 30. Queensland Department of Primary Indus tries, 14 p.
Moldgate, D. P. (1977). Propagation of ornamentals by tissue culture. In: Plant Cell. Tissue and Organ Culture, edited by J. Reinert and Y. P. S. Bajaj. Springer Verlag, Berlin. p 44-65.
Pouldan, N. (1963). Virus-free plants of chrysanthemum from approved varieties established by means of heat treatment and meristem-tip culture. Tiddskr Plan teavl 77: 689-696.
Quak, F. (1977). Meristem Culture and Virus-free Plant. In: Plant Cell, Tissue and Organ Culture, edited by J. Reinert and Y. P. S. Bajaj. Springer Verlag, Berlin. p. 598 615.
Walkey, D. G. A. (1980). Production of Virus-free Plants by Tissue Culture. In: Tissue Culture Methods for Plant Pathologists, edited by D. S. Ingram and J. P. Helgeson. Blackwell Scientifics Publications, Oxford. p. 109-117. Insert
