Effects of Plant Growth Regulators and Light Quality on <I>In Vitro </I>Seed Germination of <I>Habenaria rhodocheila</I>Hance.
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Abstract
Habenaria rhodocheila Hance. is one of the orchids which shows low seed germination even though culturing in vitro. The aim of this study was to improve seed germination of H. rhodocheila. Two sets of experiments were conducted. Firstly, the effect of pre-treatment on seeds with plant growth regulators at different concentrations using α - naphthaleneacetic acid (NAA), 6 - benzylaminopurine (BAP), and ethephon for 24 hours. After pre-treatment, the seeds were cultured on Malmgren modified terrestrial orchid medium (MM). The result showed that 0.1 mg L-1 NAA, 1.0 mg L-1 BAP, 10.0 mg L-1, and 1000.0 mg L-1 ethephon significantly promoted seed germination compared with control (free - plant growth regulator) and other treatments. Secondly, light quality treatments, seeds were sown on Murashige and Skoog medium (MS) and they were kept under six different wavelengths of light including control (under darkness). After two months of culturing, the seeds under darkness, violet (405 nm), green (546 nm), and yellow (595 nm) lights showed significantly high total seed germination than far - red light.
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