<I>In Vitro</I> Seed Culture of <I>Dendrobium peguanum</I> Lindl.
Article Sidebar
Main Article Content
Abstract
Dendrobium peguanum Lindl. is a small orchid that distributes in India, Nepal, Myanmar, and Thailand. This research aimed to use tissue culture technique with some plant growth regulators for in vitro propagation. After pollination, seed pods of D. peguanum Lindl. were harvested at 6, 8, 10 and 12 week. Seeds viability were evaluated by lacto - propionic orcein staining, and then they were culture on Murashige and Skoog medium (MS) without plant growth regulator. It was found that 6 - week - old seed pods could not germinate while those at 12 - week - old could germinate well. After that, seedlings were subcultured onto ½ MS medium containing some plant growth regulators i.e. α - naphthalene acetic acid (NAA), 6 - benzylaminopurine (BA), kinetin, and coconut water. It was found that plants grown on media with NAA 0.5 and 1.0 ppm at 120 days had better growth in term of height than other treatments, but number of shoot per plant was not differ.
Article Details
References
Basker, S. and V. Narmatha Bai. 2010. In vitro propagation of an epiphytic and rare orchid Eria bambusifolia Lindl. Research in Biotechnology 1: 15-20.
Das, M.C., S. Kumaria and P. Tandon. 2008. In vitro Propagation and Conservation of Dendrobium lituiflorum Lindl Through Protocorm-like Bodies. Journal of Plant Biochemistry and Biotechnology 17(2): 177-180.
Inwong, M. 2010. Intersectional crossability of Thai and commercial Dendrobium cultivar. M.S. Thesis. Chiang Mai University, Chiang Mai. 66 p. (in Thai)
Johansen, B. 1990. Incompatibility of Dendrobium (Orchidaceae). Botanical Journal of the Linnean Society 103(2): 165-196.
Kuichucheep, A. 2011. Utilizing coconut water mixed bio - extracted water as plant supplements for soybean production. M.S. (Environmental Management) Thesis. National Institute of Development Administration, Bankok. 170 p. (in Thai)
Kumar, P., G.S. Rawat and H.P. Wood. 2011. Diversity and Ecology of Dendrobiums (Orchidaceae) in Chotanagpur Plateau, India. Taiwania 56(1): 23-36
Murashige, T. and F. Skoog. 1962. A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures. Physiologia Plantarum 15: 473-497.
Parthibhan, S., M.V. Rao and T.S. Kumar. 2015. In vitro regeneration from protocorms in Dendrobium aqueum Lindley - An imperiled orchid. Journal of Genetic Engineering and Biotechnology 13(2): 227-233.
Pinheiro, F., D. Cafasso, S. Cozzolino and G. Scopece. 2015. Transitions between self-compatibility and self-incompatibility and the evolution of reproductive isolation in the large and diverse tropical genus Dendrobium (Orchidaceae). Annals of Botany 116(3): 457-467.
Piyatrakul, P. and P. Apavatjrut. 2004. Affect of Pod Age on Seed Germination of Habenaria rhodocheila Hance in Aseptic Conditions. Journal of Agriculture 20(3): 230-235. (in Thai)
Pradhan, S. and B. Pant. 2009. In vitro seed germination in Cymbidium elegans Lindl. and Dendrobium densiflorum Lindl. ex Wall. (Orchidaceae). Journal of Plant Science 6: 100-102.
Prasertsongskun, S. and S. Ma. 2009. Effect of plant growth regulator on plant tissue culture of Dendrobium hercoglossum Rchb.f. Journal of Yala Rajabhat University 4(2): 85-91. (in Thai)
Ramesh, T., P. Renganathan and M. Prabhakaran. 2019. In vitro studies on Dendrobium fimbriatum Hk.F - An endangered orchid. Journal of Applied and Advanced Research 4(4): 100- 103.
Santarunai, N. 2015. Effect of Medium and Plant Growth Regulator on Development and (Dendrobium friedericksianum Rchb. f.) of Orchid In vitro. The National Conference and Research Presentation 2: 155-162. (in Thai)
Subthira,T., S. Suntaranond and S. Bunnag. 2013. Effects of plant growth regulator on in vitro culture of Dendrobium aphyllum (Roxb.). KKU Research Journal (Graduate Studies) 13(1): 1-13. (in Thai)
Supinrach, S. and I. Supinrach. 2014. Effects of IBA and NAA on Rooting for the Orchid Plantlet Rhynchostylis gigantea (Lindl.) Ridl. ‘Cartoon’. Thai Science and Technology Journal 22(4): 507-514. (in Thai)
Surapha, K., K. Sareetid, M. Srinongnooch, T. Sri-in and K. Tangjitman. 2017. Effect of NAA (Naphthalene Acetic Acid) and IBA (Indolebutyric Acid) concentration on rooting of Grammatophyllum specinocum BL. shoot in Vitro. KRU National Academic Conference 5: 166-173. (in Thai)
Tipchote, A. and A. Kongbangkerd. 2010. Effect of sericin on growth and development of protocorm of Grammatophyllum speciosum Bl. NU International Journal of Science 6(2): 221-229. (in Thai)
Udomdee, W., P.J. Wen, C.Y. Lee, S.W. Chin and F.C. Chen. 2014. Effect of sucrose concentration and seed maturity on in vitro germination of Dendrobium nobile hybrids. Plant Growth Regulation 72(3): 249-255.
Wongnan, O. 2010. Intersectional crossability of some Cymbidium species. M.S. Thesis. Chiang Mai University, Chiang Mai. 85 p. (in Thai)
