Screening of Actinobacteria from Coconut Plantation Soils with Plant Growth Promotion Properties
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Abstract
Coconut is an important economic crop in Thailand. In addition to the soil organic matter, microorganisms living in soil also play an important role in plant growth promotion by enhancing mineral absorption and controlling plant - pathogenic microorganisms. The aims of this study were to isolate actinobacteria from aromatic (Nam - Hom) coconut plantation soil and investigated their plant growth promotion properties. The results showed that a total of 38 actinobacteria were obtained from the coconut plantation soil of Pran Buri, Prachuap Khiri Khan (20 isolates) and Bang Khonthi, Samut Songkhram (18 isolates). All isolates were screened for plant growth promotion properties. The results revealed that 10 isolates were found to solubilize phosphate, 25 and 17 isolates showed siderophore and indole acetic acid production, respectively. Furthermore, actinobacteria 18 and 19 isolates could produce enzyme degrade cellulose and chitin on tested - medium, respectively. Isolates R17 - 116, R17 - 210 and R17 - 208 showed high potentials of plant growth promotion features. They could convert insoluble phosphorus into soluble forms that can easily be assimilated by plants. Furthermore, they could produce siderophore which is an iron - bound substance capturing iron from soil and could produce indole acetic acid, plant hormone of auxin class. These 3 isolates will be selected for further study and for application in agriculture.
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References
Alori, E.T., B.R. Glick and O.O. Babalola. 2017. Microbial phosphorus solubilization and its potential for use in sustainable agriculture. Frontiers in Microbiology 8: 971, doi: 10.3389/fmicb.2017.00971.
Bérdy, J. 2005. Bioactive microbial metabolites. Journal of Antibiotics 58(1): 1-26.
Chaiharn, M., W. Pathom-aree, N. Sujada and S. Lumyong. 2018. Characterization of phosphate solubilizing streptomyces as a biofertilizer. Chiang Mai Journal of Science 45(2): 701-716.
Eccles, W.J., C. Matthew and A.C.P. Chu. 1990. Response of Matua prairie grass and Ellett perennial ryegrass to excess soil moisture in sand, silt and clay soils. Proceedings of the New Zealand Grassland Association 51: 127-130.
González-Franco, A.C., L. Robles-Hernández and J.L. Strap. 2017. Chitinase, chitosanase, and antifungal activities from thermophilic streptomycetes isolated from compost. International Journal of Experimental Botany 86: 14-27.
Ghorbani-Nasrabadi, R., R. Greiner, H.A. Alikhani, J. Hamedi and B. Yakhchali. 2013. Distribution of actinomycetes in different soil ecosystems and effect of media composition on extracellular phosphatase activity. Journal of Soil Science and Plant Nutrition 13(1): 223-236.
Gordon, S.A. and R.P. Weber. 1951. Colorimetric estimation of indoleacetic acid. Plant Physiology 26(1): 192-195.
Goudjal, Y., O. Toumatia, N. Sabaou, M. Barakate, F. Mathieu and A. Zitouni. 2013. Endophytic actinomycetes from spontaneous plants of Algerian Sahara: indole-3-acetic acid production and tomato plants growth promoting activity. World Journal of Microbiology and Biotechnology 29(10): 1821-1829.
Gupta, A., M. Gopal and G.V. Thomas. 2016. Beneficial microorganisms in coconut based cropping/farming systems. pp. 9. In: Book of Abstracts of National Seminar on Plantation Based Cropping System for Improving Livelihood Security. ICAR-CPCRI, Kerala, India.
Himaman, W., A. Thamchaipenet, W. Pathom-aree and K. Duangmal. 2016. Actinomycetes from Eucalyptus and their biological activities for controlling Eucalyptus leaf and shoot blight. Microbiological Research 188-189: 42-52.
Hopkinson, B.M. and F.M.M. Morel. 2009. The role of siderophores in iron acquisition by photosynthetic marine microorganisms. Biometals 22: 659-669.
Jog, R., M. Pandya, G. Nareshkumar and S. Rajkumar. 2014. Mechanism of phosphate solubilization and antifungal activity of Streptomyces spp. isolated from wheat roots and rhizosphere and their application in improving plant growth. Microbiology 160: 778-788.
Küster, E. and S.T. Williams. 1964. Selection of media for isolation of streptomycetes. Nature 202: 928-929.
Olanrewaju, O.S. and O.O. Babalola. 2019. Streptomyces: implications and interactions in plant growth promotion. Applied Microbiology and Biotechnology 103: 1179-1188.
Pikovskaya, R.I. 1948. Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Mikrobiologiya 17: 362-370.
Salwan, R. and V. Sharma. 2020. Molecular and biotechnological aspects of secondary metabolites in actinobacteria. Microbiological Research 231: 126374, doi: 10.1016/j.micres.2019.126374.
Sameera, B., H. Prakash and M.S. Nalini. 2018. Indole acetic acid production by the actinomycetes of coffee plantation soils of western ghats. International Journal of Current Research 10(10): 74482-74487.
Sato, K., Y. Kato, G. Taguchi, M. Nogawa, A. Yokota and M. Shimosaka. 2009. Chitiniphilus shinanonensis gen. nov., sp. nov., a novel chitin-degrading bacterium belonging to Betaproteobacteria. The Journal of General and Applied Microbiology 55(2): 147-153.
Schwyn, B. and J.B. Neilands. 1987. Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry 160(1): 47-56.
Sharma, S.B., R.Z. Sayyed, M.H. Trivedi and T.A. Gobi. 2013. Phosphate solubilizing microbes: Sustainable approach for managing phosphorus deficiency in agricultural soils. SpringerPlus. 2: 587, doi: 10.1186/2193-1801-2-587.
Shirling, E.B. and D. Gottlieb. 1966. Methods for characterization of Streptomyces species. International Journal of Systematic and Evolutionary Microbiology 16(3): 313-340.
Syers, J.K., A.E. Johnston and D. Curtin. 2008. Efficiency of Soil and Fertilizer Phosphorus use. Food and Agriculture Organization of the United Nations, Rome. 109 p.
Vyas, P. and A. Gulati. 2009. Organic acid production in vitro and plant growth promotion in maize under controlled environment by phosphate-solubilizing fluorescent Pseudomonas. BMC Microbiology 9: 174, doi: 10.1186/1471-2180-9-174.
Yan, N., P. Marschner, W. Cao, C. Zuo and W. Qin. 2015. Influence of salinity and water content on soil microorganisms. International Soil and Water Conservation Research 3(4): 316-323.
Zhao, Y., C. Song, H. Dong, Y. Luo, Y. Wei, J. Gao, Q. Wu, Y. Huang, L. An and H. Sheng. 2018. Community structure and distribution of culturable bacteria in soil along an altitudinal gradient of Tianshan Mountains, China. Biotechnology and Biotechnological Equipment 32(2): 397-407.